Determination of ofloxacin in human plasma using high-performance liquid chromatography and fluorescence detection

Journal of Chromatography. B, Biomedical Applications
J Macek, P Ptácek

Abstract

A new method for the determination of ofloxacin in human plasma was developed. Plasma proteins were precipitated with acetonitrile, the supernatant concentrated and injected into a reversed-phase C18 column. Enoxacin was used as an internal standard. The fluorimetric detection was performed at 282 nm for excitation and 450 nm for emission. Limit of quantitation was 20 ng/ml and the calibration curve was linear up to 6900 ng/ml.

Citations

Oct 31, 2008·Talanta·F BelalA M Al-Obaid
May 23, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hairui LiangKevin M Sowinski
Aug 22, 2000·Antimicrobial Agents and Chemotherapy·P M FurneriG Tempera
Oct 25, 2011·Journal of Pharmaceutical and Biomedical Analysis·Sandra A MeredithLubbe Wiesner
Feb 24, 2009·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Sevgi Tatar Ulu
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