Determination of optical properties of human brain tumor tissues from 350 to 1000 nm to investigate the cause of false negatives in fluorescence-guided resection with 5-aminolevulinic acid

Journal of Biomedical Optics
Norihiro HondaKunio Awazu

Abstract

The optical properties of human brain tumor tissues, including glioblastoma, meningioma, oligodendroglioma, and metastasis, that were classified into "strong," "vague," and "unobservable" fluorescence by a neurosurgeon were measured and compared. The optical properties of the tissues were measured with a double integrating sphere and the inverse Monte Carlo technique from 350 to 1000 nm. Using reasons of ex-vivo measurement, the optical properties at around 420 nm were potentially affected by the hemoglobin content in tissues. Significant differences were not observed between the optical properties of the glioblastoma regions with "strong" and "unobservable" fluorescence. Sections of human brain tumor tissue with "strong" and "unobservable" fluorescence were stained with hematoxylin and eosin. The cell densities [mean ± standard deviation (S.D.)] in regions with "strong" and "unobservable" fluorescence were 31  ±  9  ×  102  per mm2 and 12  ±  4  ×  102  per mm2, respectively, which is a statistically significant difference. The higher fluorescence intensity is associated with higher cell density. The difference in cell density modified the scattering coefficient yet it does not lead to significant differences in the reduced sc...Continue Reading

References

Jan 1, 1985·Photochemistry and Photobiology·L O Svaasand, R Ellingsen
Jul 1, 1995·Computer Methods and Programs in Biomedicine·L WangL Zheng
Jun 2, 2004·Nature Reviews. Cancer·J Martin Brown, William R Wilson
Apr 22, 2008·Neurosurgery·Walter StummerUNKNOWN ALA-Glioma Study Group
Dec 29, 2010·Advances in Pharmacological Sciences·Toshihisa IshikawaToshihiko Kuroiwa
Nov 16, 2012·Scientific Reports·Pablo A ValdésDavid W Roberts
May 12, 2015·International Journal of Molecular Sciences·Samy Eljamel
Sep 1, 2017·Biomedical Optics Express·Pablo A ValdesSylvain Gioux

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Citations

Oct 28, 2019·Biomedical Optics Express·Elina A GeninaValery V Tuchin

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