Nov 1, 1975

Determination of optimal conditions for the electron-cytochemical detection of ATPase activity in isolated nuclei

Ukrainskiĭ biokhimicheskiĭ zhurnal
O L Konoplytska, N P Sytnyanska

Abstract

The optimal conditions are selected for electron-cytochemical detection of the ATPase activity in nuclei of the skeletal muscles of rabbits and nuclei of Vicia faba L. meristem. It is shown that the previous fixation of nuclei in the rabbit skeletal muscle for 10 min in a mixture of the buffer solutions of 4% glutaric dialdehyde and 4% neutral formalin (1:1) causes a decrease in their ATPase activity by 78% in the medium containing Mg2+ and by 34% - in the medium containing Ca2+; in nuclei of horse bean seedlings meristem it lowers respectively by 28 and 16%. Ions of lead in a concentration of 0.4 mM evoke a decrease in the ATPase activity in the medium containing Mg2+, in nuclei of the rabbit skeletal muscles by 35% and in nuclei of horse bean meristem by 15% in the medium containing Ca2+. The vaule of the residual activity is sufficient for detection of the product of ATP enzymic hydrolysis reaction by activity is sufficient for detection of the product of ATP enzymic hydrolysis reaction by the method of electronic cytochemistry. An increase in the Pb2+ concentration higher than 2.8 mM evokes nonenzymic hydrolysis of ATP. The ATPase activity under the electron-cytochemical study is found within the range of pH 6.3-8.5. The pr...Continue Reading

  • References
  • Citations

References

  • We're still populating references for this paper, please check back later.
  • References
  • Citations

Citations

  • This paper may not have been cited yet.

Mentioned in this Paper

Formalin
Calcium
Meristem
Sample Fixation
Cell Nucleus
Chinchilla Rabbits
Electron Microscopy
Muscle
Canavalia ensiformis
DNA-dependent ATPase

About this Paper

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Head And Neck Squamous Cell Carcinoma

Squamous cell carcinomas account for >90% of all tumors in the head and neck region. Head and neck squamous cell carcinoma incidence has increased dramatically recently with little improvement in patient outcomes. Here is the latest research on this aggressive malignancy.

Signaling in Adult Neurogenesis

Neural stem cells play a critical role in the production of neuronal cells in neurogenesis is of great importance. Of interest is the role signalling mechanisms in adult neurogenesis. Discover the latest research on signalling in adult neurogenesis.

Psychiatric Chronotherapy

Psychiatric Chronotherapy considers the circadian rhythm as a major factor for optimizing therapeutic efficacy of psychiatric interventions. Discover the latest research on Psychiatric Chronotherapy here.

Bone Marrow Neoplasms

Bone Marrow Neoplasms are cancers that occur in the bone marrow. Discover the latest research on Bone Marrow Neoplasms here.

IGA Glomerulonephritis

IgA glomerulonephritis is a chronic form of glomerulonephritis characterized by deposits of predominantly Iimmunoglobin A in the mesangial area. Discover the latest research on IgA glomerulonephritis here.

Cryogenic Electron Microscopy

Cryogenic electron microscopy (Cryo-EM) allows the determination of biological macromolecules and their assemblies at a near-atomic resolution. Here is the latest research.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.