Determination of ortho-phenylphenol in human urine by gas chromatography-mass spectrometry

Journal of Chromatography. B, Biomedical Sciences and Applications
M J BartelsG A Bormett

Abstract

A sensitive gas chromatographic-mass spectrometric method was developed to quantitate total o-phenylphenol (OPP) (free plus conjugates) in human urine. Conjugates of OPP were acid-hydrolyzed to free OPP, derivatized to the pentafluorobenzoyl ester derivative and analyzed via negative-ion chemical ionization gas chromatography-mass spectrometry. Two stable isotope analogs of OPP were shown to be suitable as internal standards for this method (D2-phenol ring, 13C6-phenyl ring). A synthetic method is presented for the preparation of the D2-OPP internal standard. The 13C6-OPP analog was also shown to be useful as an alternate test material for laboratory-based exposure studies. The limit of quantitation for this method was 1 ng OPP/ml urine. Calibration curves were linear for the analyte over the concentration range of 0.5-1117 ng OPP/ml urine. Relative recovery of OPP from urine ranged from 97.0 to 104.7%. Low levels of OPP (mean=6+/-7 ng/ml; n=22) were found in control human urine samples. The method was validated with urine samples obtained from human volunteers undergoing a dermal exposure study with 12C-/13C6-/14C-OPP. This method was developed to aid in assessments of human exposure to OPP during a variety of uses of the comp...Continue Reading

References

May 1, 1977·Toxicology and Applied Pharmacology·S Z Cagen, J E Gibson
Dec 29, 1989·Journal of Chromatography·M Balíková, J Kohlícek
Nov 1, 1984·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·K Hiraga, T Fujii
May 11, 1984·Journal of Chromatography·R E ClineL L Needham

❮ Previous
Next ❯

Citations

Feb 12, 2011·Environmental Health Perspectives·Frances OrtonAndreas Kortenkamp
Aug 3, 2006·Journal of Agricultural and Food Chemistry·Mehmet CoelhanA Lynn Roberts

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.