Determination of oxytetracycline in blood serum by high-performance liquid chromatography with direct injection

Journal of Chromatography
R UenoT Aoki


A direct injection analysis by high-performance liquid chromatography has been developed for oxytetracycline in serum of animals and fish. A Hisep shielded hydrophobic phase column (15 cm x 4.6 mm I.D.) and a mobile phase of methanol-0.2 M oxalic acid (10:90, v/v, pH 7.0) with ultraviolet detection at 360 nm were used. The standard calibration curves in serum of chicken, hog, cattle and rainbow trout were linear over the range 0.1-20 micrograms/ml. The recoveries of oxytetracycline from all serum samples determined at two different concentrations (0.5 and 2.0 micrograms/ml) were 88-103%. The detection limit was 0.05 micrograms/ml for every serum sample.


Jul 12, 1996·Journal of Chromatography. B, Biomedical Applications·M D SantosF Haesebrouck
Jan 31, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·S SouverainJ-L Veuthey
Jul 15, 2000·Journal of Chromatography. a·H OkaH Matsumoto
Aug 10, 2000·Journal of Pharmaceutical and Biomedical Analysis·I N PapadoyannisL A Kovatsi
Dec 6, 2003·Journal of Pharmaceutical and Biomedical Analysis·C Pistos, James T Stewart
Sep 22, 2016·Critical Reviews in Analytical Chemistry·Rúbia Adrieli SversutHérida Regina Nunes Salgado

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