Determination of parvovirus antigen in the vaccine using time-resolved fluorescence immunoassay.

Biotechnology and Applied Biochemistry
Cuicui ChenLaiqing Li

Abstract

As a highly contagious and potentially fatal disease of dogs, canine parvovirus type 2 (CPV-2) usually causes severe myocarditis and gastroenteritis, while vaccine injection has greatly reduced the incidence of CPV-2 diseases. However, there is currently a lack of simple and effective method for quantitative detection of CPV-2 in vaccine. Therefore, this study aims to prepare an accurate method to determine the CPV-2 antigen (CPV-2-Ag) in vaccine. Here, a sandwich time-resolved fluorescence immunoassay (TRFIA) was established and optimized. Anti-CPV-2 antibodies were immobilized on 96-well plates to capture CPV-2-Ag, and then bound together with the detection antibodies labeled with Europium(III) (Eu3+ ) chelates; finally, time-resolved fluorometry was employed to measure the fluorescence intensity. Vaccination was performed to evaluate the relationship between CPV-2-Ag concentration and antibody titer. The sensitivity is 1.15 mEU/mL (LogY = 1.524 + 0.8667 × LogX, R2  = 0.9933), and the average recovery is among 91.00% to 106.39% without cross-reactions with the other canine viral antigen. The correlation between ELISA assay and this method is up to 0.9861. And, there is high correlation between the CPV-2-Ag concentration and a...Continue Reading

References

Jun 19, 2002·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·D S Silin, V Lyubomska
Dec 3, 2002·Journal of the American Veterinary Medical Association·Donald J KlingborgCharles R Short
Jun 23, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Trevor WanerEphraim Keren-Kornblatt
Feb 27, 2007·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Kader MuhamudaVasanthapuram Ravi
Aug 13, 2011·The Journal of Emergency Medicine·Richard T GriffeyRobert F Poirier
Jan 26, 2013·The Analyst·Ju-Young ByunMin-Gon Kim
Jan 23, 2016·Veterinary Microbiology·Carla MirandaGertrude Thompson
Jul 4, 2017·Journal of the Science of Food and Agriculture·Xiaofei HuGaiping Zhang
May 1, 2019·Biotechnology and Applied Biochemistry·Cuicui ChenLaiqing Li

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