PMID: 2102265Jan 1, 1990Paper

Determination of penicillin in pharmaceutical formulations by flow injection analysis using an optimised immobilised penicillinase reactor and iodometric detection

Journal of Pharmaceutical and Biomedical Analysis
M A van OpstalA Bult

Abstract

An automated assay for the determination of penicillin in formulations suitable for use in pharmaceutical quality control is presented. The method is based on the classical iodometric penicillin assay which is incorporated in a flow injection analysis (FIA) system. The required hydrolysis is performed on-line by using an immobilised penicillinase reactor. Packed-bed and single-bead-string enzyme reactors are compared. It turns out that a packed-bed penicillinase reactor (10 cm x 1.5 mm i.d.) provides complete hydrolysis within short residence time, while only little back-pressure is generated. This enzyme reactor is stable for at least 9 months. Enzymatic hydrolysis of the beta-lactam ring results in the formation of the corresponding penicilloic acid, which consumes iodine. The iodine consumption is determined colorimetrically by measuring the decrease of the absorbance of the blue coloured iodine/starch complex. The optimum reactor length and flow rate for the colourimetrical detection reaction are determined. The optimised method is applied to the assay of penicillin in formulations and the results are compared with the "true" results obtained with a reference method: a mercurimetric titration. The reliability of the flow in...Continue Reading

References

Feb 1, 1963·The Journal of Pharmacy and Pharmacology·J M HAMILTON-MILLERR KNOX
Jan 1, 1988·The Journal of Automatic Chemistry·J RuzM D de Castro

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Citations

Sep 15, 2006·Journal of Separation Science·Victoria F SamanidouIoannis N Papadoyannis
Jul 27, 2012·Journal of Neural Engineering·Owen FalzonJoseph Muscat

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