Abstract
The potential for the use of the disk diffusion method to accurately predict penicillin MICs for Streptococcus pneumoniae was investigated with penicillin (6 microg), methicillin (5 microg), and oxacillin (1 microg) disks. A total of 183 S. pneumoniae isolates were tested by three MIC procedures (agar dilution, microdilution, and E-test). Regression analyses of the geometric mean of the three MIC results against (i) the sum of the zone diameters for methicillin, penicillin, and oxacillin disks; (ii) the sum of the zone diameters for methicillin and penicillin disks; and (iii) each of the three individual zone diameters were performed. Calculated MICs were determined from each of these regression analyses and compared to the mean reference MICs. A high level of correlation was obtained with both the two- and the three-disk procedures (r = 0.97), with essential agreement rates (+/-1 doubling dilution) between MICs calculated by the three-disk procedure and the two-disk procedure and the mean reference MICs of 98.4 and 98.9%, respectively. No major or very major errors were obtained with the two- or three-disk procedures. The accuracy of the disks used individually was lower (r = 0.84 to 0.93). However, oxacillin and methicillin d...Continue Reading
References
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Citations
Sep 1, 2011·Foodborne Pathogens and Disease·Karin HoelzerMartin Wiedmann
Oct 1, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Rosemol Varghese, Balaji Veeraraghavan
May 29, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Peter C Appelbaum
Sep 24, 2020·Antibiotics·Adam KmeckGregory R Wiedman
May 23, 2000·The Pediatric Infectious Disease Journal·M R Jacobs
Jun 19, 2018·The Journal of Antimicrobial Chemotherapy·Stefano ManciniErik C Böttger
Apr 20, 1999·Journal of Chemotherapy·M R Jacobs
Jan 4, 2001·Journal of Clinical Microbiology·F C TenoverR Williams