Determination of Permeability Coefficients of Polymersomal Membranes for Hydrophilic Molecules

Langmuir : the ACS Journal of Surfaces and Colloids
Sarah T PoschenriederKathrin Castiglione

Abstract

Polymer vesicles, so-called polymersomes, can be applied as carrier-systems and universal reaction compartments, due to the possibility to encapsulate guest molecules. Compared to common lipid vesicles, polymersomes show an increased stability and decreased membrane permeability. Control of the mass transport across the membrane is necessary for any application, requiring the precise knowledge of the permeability. So far, data on permeability coefficients of polymersomal membranes are scarce because commonly applied release assays are confronted with the challenge of high detection limits and alternative methods developed so far are either restricted to the use of a certain permeating molecule or rely on the use of nuclear magnetic resonance measurements. In contrast, an influx assay that is broadly applicable to hydrophilic molecules and does not involve specialized equipment was developed in this work, which is based on the passive diffusion of compounds into initially empty vesicles. The method is valid for hydrophilic molecules that show no membrane retention and, thus, do not accumulate within the membrane. Using this method, the permeability of polymersomes made of poly(2-methyloxazoline)15-poly(dimethylsiloxane)68-poly(2...Continue Reading

References

Sep 17, 1968·Biochimica Et Biophysica Acta·R E WoodH E Morgan
Sep 1, 1993·Chemistry and Physics of Lipids·A D Bangham
Feb 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·G A BrooksC E Butz
May 15, 1999·Science·B M DischerD A Hammer
Oct 31, 2001·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·A AvdeefI Khan
Jan 5, 2002·Applied Microbiology and Biotechnology·Y LiS Y Lun
Mar 20, 2002·Current Topics in Medicinal Chemistry·A Avdeef
Aug 10, 2002·Science·Dennis E Discher, Adi Eisenberg
Mar 5, 2004·Chembiochem : a European Journal of Chemical Biology·Sarah J LuchanskyCarolyn R Bertozzi
Apr 6, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Fariyal Ahmed, Dennis E Discher
Apr 29, 2004·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Per E Nielsen, Alex Avdeef
Jun 29, 2004·Journal of Biotechnology·Noppol LeksawasdiPeter L Rogers
Jul 23, 2004·The Journal of Chemical Physics·Anja RumpleckerChristian Mayer
Dec 14, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Fenghua MengJan Feijen
Jan 18, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Pavel BrozPatrick Hunziker
Feb 15, 2005·Proceedings of the National Academy of Sciences of the United States of America·P Peter GhoroghchianMichael J Therien
Feb 16, 2006·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Alex Avdeef, Oksana Tsinman
May 17, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Giuseppe BattagliaSalvador Tomas
Jul 13, 2006·Annual Review of Biomedical Engineering·Dennis E Discher, Fariyal Ahmed
Oct 20, 2006·European Biophysics Journal : EBJ·Heiko Heerklotz, Joachim Seelig
Feb 7, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Alexander WittemannAdi Eisenberg
Apr 10, 2007·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Holger FischerFrank Senner
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Manish KumarWolfgang Meier
Jan 2, 2008·The Journal of General Physiology·John C MathaiMark L Zeidel
Jun 24, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Zhiliang Cheng, Andrew Tsourkas
Jan 7, 2009·Biomacromolecules·Fenghua MengJan Feijen
Apr 18, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Caroline De VochtJan Steyaert
Apr 23, 2009·Pharmaceutical Research·S LitvinchukW Meier
Aug 24, 2010·Environmental Science & Technology·Erica L DiFilippo, Robert P Eganhouse
Feb 22, 2011·The European Physical Journal. E, Soft Matter·J-F Le MeinsS Lecommandoux
Mar 17, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Autumn CarlsenSébastien Lecommandoux
Dec 31, 2011·Bioresource Technology·Peter Riedlberger, Dirk Weuster-Botz
Jan 14, 2012·Biointerphases·Madhavan NallaniWalter Hunziker
Sep 27, 2012·Bioprocess and Biosystems Engineering·Peter RiedlbergerDirk Weuster-Botz
Oct 2, 2012·Angewandte Chemie·Linge WangGiuseppe Battaglia
Mar 26, 2015·Protein Expression and Purification·Ludwig KlermundKathrin Castiglione
Sep 1, 2015·Chemical Reviews·Jens GaitzschBrigitte Voit
Nov 14, 2015·Chemical Society Reviews·Cornelia G PalivanWolfgang Meier

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Citations

May 20, 2018·Bioprocess and Biosystems Engineering·Ludwig Klermund, Kathrin Castiglione
Jul 3, 2018·Science Advances·Jay R Werber, Menachem Elimelech
Feb 4, 2021·ACS Central Science·Alisha J MillerRachel K O'Reilly

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