Determination of Phenolic Content in Different Barley Varieties and Corresponding Malts by Liquid Chromatography-diode Array Detection-Electrospray Ionization Tandem Mass Spectrometry

Antioxidants
Daniel O CarvalhoLuís F Guido

Abstract

A simple and reliable method for the simultaneous determination of nine phenolic compounds in barley and malted barley was established, using liquid chromatography-diode array detection-electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI-MS/MS). The phenolic compounds can be easily detected with both systems, despite significant differences in sensitivity. Concentrations approximately 180-fold lower could be achieved by mass spectrometry analysis compared to diode array detection, especially for the flavan-3-ols (+)-catechin and (-)-epicatechin, which have poor absorptivity in the UV region. Malt samples were characterized by higher phenolic content comparing to corresponding barley varieties, revealing a significant increase of the levels of (+)-catechin and (-)-epicatechin during the malting process. Moreover, the industrial malting is responsible for modification on the phenolic profile from barley to malt, namely on the synthesis or release of sinapinic acid and epicatechin. Accordingly, the selection of the malting parameters, as well as the barley variety plays an important role when considering the quality and antioxidant stability of beer.

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Citations

Oct 30, 2016·International Journal of Food Sciences and Nutrition·Alessandra BordoniVeronica Valli
May 5, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sherif T S HassanKateřina Berchová-Bímová
Mar 11, 2020·Foods·Daniel RicoAna B Martín-Diana
Jan 24, 2018·Journal of Food Science and Technology·Mehmet Musa ÖzcanNurhan Uslu
Aug 16, 2019·Journal of Food Science and Technology·Dániel KorenGabriella Kun-Farkas
Aug 30, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Justyna GąsiorAlicja Z Kucharska
Apr 23, 2020·Oxidative Medicine and Cellular Longevity·Yawen ZengJiazhen Yang
Dec 15, 2020·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Irene A RetmanaRolf W Sparidans
Jul 1, 2018·Comprehensive Reviews in Food Science and Food Safety·Julia WannenmacherThomas Becker
Oct 31, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sanja RadonjićTatjana Košmerl
May 21, 2021·Critical Reviews in Food Science and Nutrition·Huiqin GuoZhuoyu Li

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Methods Mentioned

BETA
glycosylation

Software Mentioned

Chrompass
Xcalibur
Excel

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