Determination of phosphatidylglycerol in amniotic fluid for prediction of foetal lung maturity by microbore-column liquid chromatography

Journal of Chromatography
T IshizukaT Tsuda

Abstract

Phosphatidylglycerol (PG) has been determined in amniotic fluid from 55 patients by using a microbore-column liquid chromatographic system. The present analysis time is 1 h 40 min for pretreatment of amniotic fluid and 20 min for a chromatographic run. From 2 ml of amniotic fluid, the PG content has been determined between 1.0 and 0.05 mg/dl. The detection limit of PG is 10 ng. As the injection volume (0.5 microliter) is small, repeated analyses are possible if necessary. It is concluded that, in the case of PG values over 0.10 mg/dl, a mother can deliver an infant without respiratory distress syndrome. This method is useful not only for the prenatal evaluation of lung maturity, but also for the assessment of any therapeutic effect.

References

Dec 15, 1979·American Journal of Obstetrics and Gynecology·M ObladenL Gluck
Nov 11, 1977·Journal of Chromatography·W M Hax, W S van Kessel
Feb 15, 1973·American Journal of Obstetrics and Gynecology·L Gluck, M V Kulovich
Feb 1, 1971·American Journal of Obstetrics and Gynecology·L GluckW N Spellacy
Sep 15, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·R D PatonR W Logan

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