Determination of piceatannol in rat serum and liver microsomes: pharmacokinetics and phase I and II biotransformation

Biomedical Chromatography : BMC
Kathryn A RoupeNeal M Davies

Abstract

A method of analysis of piceatannol in biological fluids is necessary to study the kinetics of in vitro and in vivo metabolism and determine its concentration in foodstuffs. A novel and simple high-performance liquid chromatographic method was developed for simultaneous determination of piceatannol and products of its metabolism in rat serum and liver microsomes. Serum, or microsomes (0.1 mL), were precipitated with acetonitrile after addition of the internal standard, 4-methylumbelliferone. Separation was achieved on a phenomenex C(18) column (250 x 4.6 mm i.d., 5 microm) equipped with a phenomenex C(18) (4 x 3.0 mm i.d., 5 microm) guardcolumn with fluorescence excitation at 320 nm and emission at 420 nm. Separation was also possible with UV detection at 310 nm. The fluorescent calibration curves were linear ranging from 0.05 to 100 microg/mL. The mean extraction efficiency was >95%. Precision of the assay was <10% (coefficient of variation), and was within 10% at the limit of quantitation (0.05 ng/mL). Bias of the assay was lower than 7%. The limit of detection was 50 ng/mL for a 0.1 mL sample. The assay was applied successfully to the in vitro kinetic study of metabolism of piceatannol in rat liver microsomes and pharmacokin...Continue Reading

Citations

Mar 30, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hanane MessiadOmar Houache
Jan 14, 2016·Journal of Agricultural and Food Chemistry·Mohamed Ali SeyedKavitha Vijayaraghavan
Nov 24, 2011·Mutation Research·Hanna PiotrowskaMarek Murias
Jan 23, 2016·Oxidative Medicine and Cellular Longevity·J Antoni SirerolAngel L Ortega
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Aug 21, 2010·The Journal of Pharmacy and Pharmacology·Michaela MiksitsWalter Jäger
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Apr 8, 2017·Journal of Medicinal Food·Jonathan Kershaw, Kee-Hong Kim
Nov 30, 2006·The Journal of Pharmacy and Pharmacology·Kathryn A RoupeNeal M Davies
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