PMID: 6167673Apr 1, 1981Paper

Determination of picomole amounts of dopamine, noradrenaline, 3,4-dihydroxyphenylalanine, 3,4-dihydroxyphenylacetic acid, homovanillic acid, and 5-hydroxyindolacetic acid in nervous tissue after one-step purification on Sephadex G-10, using high-performance liquid chromatography with a novel type of electrochemical detection

Journal of Neurochemistry
B H Westerink, T B Mulder

Abstract

A determination of dopamine (DA), noradrenaline (NA), 3,4-dihydroxyphenylalanine (DOPA), 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA), and 5-hydroxyindolacetic acid (5-HIAA) in nervous tissue is described. The method is based on a rapidly performed isolation of DA, NA, DOPA, DOPAC, HVA, and 5-HIAA from one single nervous tissue sample on small columns of Sephadex G-10, followed by reverse-phase high-performance liquid chromatography with electrochemical detection. A new type of electrochemical detector based on a rotating disk electrode (RDE) was used. The rotating disc electrode was found to be a reliable and sensitive amperometric detector with several advantages over the currently used thin-layer cells. The detector appeared very useful for routine analysis. Practical details are given for the routine use of the RDE. Brain samples containing no more than 75-150 pg (DA, DOPA, DOPAC, HVA, and 5-HIAA) or 500 pg (NA) could be reproducibly assayed with high recovery (approx. 85%) and precision (approx. 5%), without the use of internal standards. Endogenous concentrations of DA, NA, DOPA, DOPAC, HVA, and 5-HIAA were determined in eight brain structures.

References

Sep 1, 1979·European Journal of Pharmacology·B H Westerink, A S Horn
Sep 1, 1979·Journal of Neurochemistry·G Zürcher, M Da Prada
Nov 23, 1979·European Journal of Pharmacology·A S HornD Dijkstra
Apr 1, 1977·Journal of Neurochemistry·J W KebabianJ Axelrod
Sep 3, 1976·Brain Research·D H VersteegM Palkovits
Jun 1, 1976·European Journal of Pharmacology·B H Westerink, J Korf

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Citations

Jun 1, 1992·Naunyn-Schmiedeberg's Archives of Pharmacology·F Pi, J A García-Sevilla
Apr 1, 1992·Acta Physiologica Scandinavica·H LagercrantzL Peyrin
Apr 1, 1986·Naunyn-Schmiedeberg's Archives of Pharmacology·M VidalS Z Langer
Apr 1, 1986·Journal of Neurochemistry·B H Westerink, R J Kikkert
May 1, 1985·The Journal of Pharmacy and Pharmacology·H RollemaA S Horn
Jan 1, 1988·Journal of Neurochemistry·D S GoldsteinR J Porter
Aug 1, 1986·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·B H Westerink, N ten Kate
Sep 1, 1983·Naunyn-Schmiedeberg's Archives of Pharmacology·M G FeenstraA S Horn
Aug 1, 1993·Pharmacology & Toxicology·J C FlórioJ Palermo-Neto
Jan 1, 1988·Movement Disorders : Official Journal of the Movement Disorder Society·J G de YebenesA Vazquez
Jan 1, 1990·Journal of Neural Transmission. General Section·O BoulatL Maitre
Jan 1, 1987·Journal of Neural Transmission·L A HilakiviM Attila
Nov 1, 1984·British Journal of Pharmacology·L G Hársing, E S Vizi
Jun 1, 1982·Journal of Neurochemistry·D J Dooley, H Bittiger
Apr 1, 1985·British Journal of Pharmacology·F BrownK Randall
Nov 1, 1982·Journal of Neurochemistry·G M Tyce, D K Rorie
Mar 1, 1986·Naunyn-Schmiedeberg's Archives of Pharmacology·P PetruzzoS Z Langer
Jan 1, 1986·Journal of Neural Transmission·R FavreL Peyrin
Jun 1, 1984·Naunyn-Schmiedeberg's Archives of Pharmacology·M G FeenstraA S Horn
Nov 1, 1988·Naunyn-Schmiedeberg's Archives of Pharmacology·S M Tejani-ButtD J Brunswick
Oct 1, 1984·Naunyn-Schmiedeberg's Archives of Pharmacology·J C van OeneA S Horn
Dec 23, 1996·The Journal of Comparative Neurology·J C HolstegeC M Bongers

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