Determination of plasma kisspeptin concentrations during reproductive cycle and different phases of pregnancy in crossbred cows using bovine specific enzyme immunoassay

General and Comparative Endocrinology
Mohan MondalBukkaraya Samudram Prakash

Abstract

Kisspeptin, a decapeptide and potent secretagogue of GnRH has been emerged recently as a master player in the regulation of reproduction in animals. Determination of kisspeptin in peripheral circulation is, therefore, very important for studying the control of its secretion and its role on reproduction in bovine species, the information on which is not available during any physiological state in this species, may probably be due to non-availability of simple assay procedure to measure the hormone. Therefore, the objective of this study was to develop and validate a simple and sufficiently sensitive enzyme immunoassay (EIA) for kisspeptin determination in bovine plasma using the biotin-streptavidin amplification system and second antibody coating technique. Biotin was coupled to kisspeptin and used to bridge between streptavidin-peroxidase and the immobilized kisspeptin antiserum in the competitive assay. The EIA was conducted directly in 100 μl of unknown bovine plasma. Kisspeptin standards ranging from 0.01 to 25.6 ng/100 μl/well were prepared in hormone-free plasma. The lowest detection limit was 0.1 ng/ml plasma. Plasma volumes for the EIA, viz., 50, 100 and 200 μl did not influence the shape of standard curve even though a ...Continue Reading

References

Mar 29, 2000·Theriogenology·L J KulickO J Ginther
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Feb 8, 2003·The Journal of Clinical Endocrinology and Metabolism·Yasuko HorikoshiMasahiko Fujino
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Mar 20, 2013·Proceedings of the National Academy of Sciences of the United States of America·Han Kyoung ChoeKyungjin Kim
Aug 16, 2014·The Journal of Clinical Endocrinology and Metabolism·C N JayasenaW S Dhillo

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Citations

Mar 8, 2017·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Junyu ZhaiZi-Jiang Chen
Sep 1, 2018·Reproduction in Domestic Animals = Zuchthygiene·Annalisa RizzoRaffaele Luigi Sciorsci
Jan 23, 2018·Frontiers in Endocrinology·Kai-Lun HuJie Qiao
Feb 28, 2020·Theriogenology·Ampika ThongphakdeeKaywalee Chatdarong

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