Determination of polychlorinated biphenyls and organochlorine pesticides in small volumes of human blood by high-throughput on-line SPE-LVI-GC-HRMS

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Jürgen WittsiepeMichael Wilhelm

Abstract

A fully automated and robust method featuring on-line solid-phase extraction (SPE) and large volume injection (LVI) gas chromatographic (GC) high resolution mass spectrometry (HRMS) is used to determine polychlorinated biphenyls (PCBs) and organochlorine pesticides, such as penta- and hexachlorobenzene (PeCBz, HxCBz), hexachlorocyclohexane isomers (HCH) and 4,4'-dichlorodiphenyldichloroethene (a metabolite of dichlorodiphenyltrichloroethane (DDT)), with only 200μl of human blood, serum or plasma. After spiking the sample with (13)C-labeled internal standards and precipitating the proteins, the sample is passed through a 10mm×2.0mm ID SPE cartridge filled with C18 material that adsorbs the analytes. After washing and drying, the cartridge is extracted with hexane/dodecane (99/1, v/v); the extract is directly injected into a LVI where GC/HRMS analysis follows. The fully automated system utilizes a robotic autosampler and a modular SPE system including two high-pressure syringe pumps, an automatic SPE cartridge exchanger unit and 6 switchable valves. All sample preparation steps are performed within 20min during the GC run of a previous sample, limiting the throughput with only the GC runtime. The contents are quantified using the...Continue Reading

References

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Jul 1, 2006·Journal of Chromatography. a·Jean-François FocantEdwin De Pauw
Oct 19, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Raul LópezEsmeralda Millán
Jan 16, 2007·Chemosphere·Jürgen WittsiepeMichael Wilhelm
Mar 31, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Buu N TranKenneth Aldous
Oct 9, 2009·Journal of Chromatography. a·Mohamed Abdel-Rehim
Dec 17, 2009·Electrophoresis·Rawi RamautarGerhardus J de Jong
Aug 2, 2011·Analytica Chimica Acta·Mohamed Abdel-Rehim

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Citations

Mar 13, 2016·Analytical and Bioanalytical Chemistry·Caroline ChataBrice M R Appenzeller
Sep 22, 2015·Analytica Chimica Acta·Vicent YusaMarta Roca
Feb 13, 2021·Critical Reviews in Analytical Chemistry·Sezin ErarpatSezgin Bakırdere
May 28, 2019·Environment International·Kathrin WolfWolfgang Rathmann

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