Determination of polycyclic aromatic hydrocarbons in food samples by automated on-line in-tube solid-phase microextraction coupled with high-performance liquid chromatography-fluorescence detection

Journal of Chromatography. a
A IshizakiH Kataoka

Abstract

A simple and sensitive automated method, consisting of in-tube solid-phase microextraction (SPME) coupled with high-performance liquid chromatography-fluorescence detection (HPLC-FLD), was developed for the determination of 15 polycyclic aromatic hydrocarbons (PAHs) in food samples. PAHs were separated within 15 min by HPLC using a Zorbax Eclipse PAH column with a water/acetonitrile gradient elution program as the mobile phase. The optimum in-tube SPME conditions were 20 draw/eject cycles of 40 microL of sample using a CP-Sil 19CB capillary column as an extraction device. Low- and high-molecular weight PAHs were extracted effectively onto the capillary coating from 5% and 30% methanol solutions, respectively. The extracted PAHs were readily desorbed from the capillary by passage of the mobile phase, and no carryover was observed. Using the in-tube SPME HPLC-FLD method, good linearity of the calibration curve (r>0.9972) was obtained in the concentration range of 0.05-2.0 ng/mL, and the detection limits (S/N=3) of PAHs were 0.32-4.63 pg/mL. The in-tube SPME method showed 18-47 fold higher sensitivity than the direct injection method. The intra-day and inter-day precision (relative standard deviations) for a 1 ng/mL PAH mixture we...Continue Reading

References

Jul 13, 2000·Journal of Chromatography. a·H KataokaJ Pawliszyn
Apr 19, 2005·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·K B Okona-MensahR Fielder
Sep 28, 2006·Journal of Agricultural and Food Chemistry·Justin Koffi HouessouValérie Camel
Jul 7, 2007·Food Additives and Contaminants·Vincent VarletCarole Prost
Feb 12, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·P ViscianoM Amorena
Aug 5, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Roser Martí-CidJosé L Domingo

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Citations

Dec 4, 2012·International Journal of Environmental Research and Public Health·Yong-Hong ChenHua-Bin Li
Aug 7, 2013·Journal of Chromatographic Science·Xiaofang LiuHuaixia Chen
Jan 17, 2016·Analytica Chimica Acta·M Fernández-AmadoD Prada-Rodríguez
Dec 30, 2015·Critical Reviews in Food Science and Nutrition·Vasudha BansalKi-Hyun Kim
Jul 24, 2015·Environment International·Vasudha Bansal, Ki-Hyun Kim
Dec 20, 2013·Journal of Chromatography. a·Yang YangJanusz Pawliszyn
Jun 13, 2015·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Afolabi AdisaMahmoud A Saleh
Jun 11, 2014·Critical Reviews in Food Science and Nutrition·Iria Yebra-PimentelJesús Simal-Gándara
May 23, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·A GomesL C Roseiro
Jan 13, 2018·Journal of Separation Science·Juanjuan FengMin Sun
Sep 4, 2013·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Charinrat SirithamProespichaya Kanatharana
Mar 19, 2020·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·B K K K JinadasaSimone Morais
Dec 19, 2012·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Sujittra PoorahongProespichaya Kanatharana
Feb 4, 2016·Asian Pacific Journal of Cancer Prevention : APJCP·Elliyana Nadia HamidiAhmad Faizal Abdull Razis

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