Feb 1, 1993

Determination of proliferative characteristics of growth plate chondrocytes by labeling with bromodeoxyuridine

Calcified Tissue International
Cornelia E Farnum, Norman J Wilsman


Postnatal bone growth occurs by the process of endochondral ossification in cartilaginous growth plates at the ends of long bones. The rate and extent of long bone growth is determined by a combination of chondrocytic proliferation, matrix production, and increase in chondrocytic height in the direction of growth during cellular enlargement. In this study, single pulse and/or repeated pulse labeling with the thymidine analog bromodeoxyuridine (BrdU) was used to study the role of cellular proliferation in controlling long bone growth. Variables studied included progression of the label over time following a pulse, and patterns and progression of the label over time following repeated pulse labeling for 24 and 48 hours. Examination was made of the proliferative characteristics of chondrocytes, the spatial pattern of cellular proliferation, and cell cycle kinetics. With respect to the spatial pattern of proliferative chondrocytes, results suggest that chondrocytes within a column are more synchronized with each other than are chondrocytes in different columns. This is consistent with the concept that each column represents a clonal expansion of a stem cell, which may proceed independently from adjacent columns. Despite this appare...Continue Reading

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Mentioned in this Paper

Endochondral Ossification
Cell Proliferation
Epiphysis of Bone
Cell Division Phases
Cell Cycle
Structure of Long Bone
Clonal Expansion

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