Determination of properties of individual liposomes by capillary electrophoresis with postocolumn laser-induced fluorescence detection

Analytical Chemistry
C F DuffyEdgar A Arriaga

Abstract

Individual liposome measurements by capillary electrophoresis with postcolumn laser-induced fluorescence detection facilitated the determination of liposome property distributions, two-dimensional plots, and an improved characterization of a liposomal preparation. This advancement in liposome analysis was feasible by using a high-sensitivity postcolumn laser-induced fluorescence detector wired for millisecond response. For each individual liposome containing fluorescein, peak height and migration time were determined. From these measurements the individual entrapped volumes and electrophoretic mobilities were determined. Distribution analysis of these properties facilitated comparison of various liposome dilutions and indicated that the method is reproducible and unaffected by the density of liposomes (10(7)-10(9) liposomes/mL) in the suspension. Furthermore, liposomes showed entrapped volumes that vary from 0.3 to 13 fL with apparent radius varying from 370 nm to 1.8 microns. Two-dimensional plots of reduced mobility versus kappa R (Debye parameter x liposome radius) revealed that the liposomes resuspended from a dried film of phospholipids are heterogeneous in regard to the surface charge density of individual liposomes. The ...Continue Reading

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Citations

Aug 24, 2004·Analytical and Bioanalytical Chemistry·Ruth KuldveeMarja-Liisa Riekkola
Dec 2, 2005·Analytical and Bioanalytical Chemistry·Hossein AhmadzadehEdgar A Arriaga
Jul 19, 2008·Annual Review of Analytical Chemistry·Daniella CohenNorman J Dovichi
Jul 1, 2017·Electrophoresis·Vojtech Adam, Marketa Vaculovicova
Dec 3, 2002·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kazuhiko TsukagoshiMizuo Maeda
Jun 26, 2008·Electrophoresis·Vratislav Kostal, Edgar A Arriaga
Jun 20, 2007·Electrophoresis·Yun ChenEdgar A Arriaga
Nov 23, 2006·Electrophoresis·Christofer E Whiting, Edgar A Arriaga
Aug 26, 2010·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Lisa MellanderAndrew G Ewing
Jun 12, 2009·Electrophoresis·Susanne K Wiedmer, Ruth Shimmo
May 19, 2010·Analytical and Bioanalytical Chemistry·Donna M OmiatekAndrew G Ewing
Aug 29, 2006·Analytical and Bioanalytical Chemistry·Ryan D JohnsonEdgar A Arriaga
May 1, 2004·Journal of Medicinal Food·Cory J Stephanson, G Patrick Flanagan
Sep 20, 2006·Electrophoresis·Gerhard BilekErnst Kenndler
Aug 4, 2007·Electrophoresis·Xiupei YangMartin M F Choi
Jul 8, 2018·International Journal of Pharmaceutics·Siyam M AnsarThilak Mudalige
Oct 5, 2020·Journal of Pharmaceutical and Biomedical Analysis·Yuchen FanKelly Zhang
Apr 6, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Michele D Pysher, Mark A Hayes
Nov 17, 2009·Analytical Chemistry·Vratislav KostalMichael T Bowser
Sep 2, 2008·Journal of Agricultural and Food Chemistry·Dion M A M LuykxHans Bouwmeester
Feb 2, 2006·Analytical Chemistry·Yun Chen, Edgar A Arriaga
Nov 30, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Jingyuan ChenTianbao Li
Dec 28, 2004·Chemistry : a European Journal·Patrick FalveyBart Jan Ravoo
Oct 26, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Angela R Eder, Edgar A Arriaga
Jan 5, 2011·Analytical Chemistry·Gregory G WolkenEdgar A Arriaga
May 25, 2007·Journal of Chromatography. a·Christofer E Whiting, Edgar A Arriaga

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