PMID: 9174287Apr 11, 1997Paper

Determination of propofol in rat whole blood and plasma by high-performance liquid chromatography

Journal of Chromatography. B, Biomedical Sciences and Applications
M H Yeganeh, I Ramzan

Abstract

A simple, accurate and sensitive high-performance liquid chromatographic method was developed for the determination of propofol, an intravenous anaesthetic agent, in rat whole blood or plasma samples. The method is based on precipitation of the protein in the biological fluid sample and direct injection of the supernatant into an HPLC system involving a C18 reversed-phase column using a methanol-water (70:30) mobile phase delivered at 1 ml/min. Propofol and the internal standard (4-tert.-octylphenol) were quantified using a fluorescence detector set at 276 nm (excitation) and 310 nm (emission). The analyte and internal standard had retention times of 6.3 and 10.5 min, respectively. The limit of quantification for propofol was 50 ng/ml using 100 microl of whole blood or plasma sample. Calibration curves were linear (r2=0.99) over a 1-10 microg/ml concentration range and intra- and inter-day precision were between 4-11%. The assay was applied to the determination of propofol whole blood pharmacokinetics and propofol whole blood to plasma distribution ratios in rats.

Citations

Jun 22, 2000·Biomedical Chromatography : BMC·A L DawidowiczA Fijalkowska
Dec 13, 2000·Biomedical Chromatography : BMC·A L Dawidowicz, E Fornal
Jan 6, 2006·Archives of Pharmacal Research·Hyun-Ki RyooEun-Seok Park
Sep 3, 2013·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Paulo PaixãoJosé A G Morais
Sep 18, 2002·Biochemical Pharmacology·Corinne LejusAndré Guillouzo
Oct 25, 2013·Archives of Pharmacal Research·Eunyoung Han
May 16, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Liqiang GuHaoyun An
Apr 12, 2008·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Hanna KumpulainenJarkko Rautio
Nov 30, 2006·Journal of Pharmaceutical and Biomedical Analysis·Xavier CussonneauRoselyne Boulieu
Jan 9, 2008·Paediatric Anaesthesia·Thomas EngelhardtBruno Bissonnette
Jun 14, 2008·British Journal of Anaesthesia·F RavenelleD C Smith
Jul 23, 1999·Biomedical Chromatography : BMC·S EmaraM A Bakr
Feb 23, 2019·Journal of Neural Engineering·Amirali ToossiVivian K Mushahwar
Nov 21, 2019·Luminescence : the Journal of Biological and Chemical Luminescence·Mona E El SharkasyM M Salim
Aug 1, 2012·Clinical Pharmacokinetics·Jeroen DiepstratenAlexander A Vinks
Apr 11, 2018·Journal of Chromatographic Science·Magda M AyadNora Elsayed

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