Determination of protein-bound palmitate turnover rates using a three-compartment model that formally incorporates [3H]palmitate recycling

Biochemistry
Riad Qanbar, Michel Bouvier

Abstract

The observation that the palmitoylation state of certain proteins can be biologically modulated led to the proposal that it could, much like phosphorylation, be an important dynamic regulator of protein function. However, based on single-phase exponential decay analysis of data from [(3)H]palmitate pulse/chase experiments, the measured protein-bound palmitate turnover rates were often found to be too slow to account for rapid physiological responses. This paper reports that exponential decay does not adequately describe the results of such experiments because it fails to account for the recycling of [(3)H]palmitate from cellular lipids to palmitoyl CoA. Taking this recycling into account, a three-compartment model was used to deduce the time-dependent changes of cellular [(3)H]palmitoyl CoA and to infer the time course for the incorporation of [(3)H]palmitate into proteins. The validity of the inferences made by the model was checked against data obtained by metabolic labeling of endogenous HEK293 cell proteins. In addition, the model could account for reported anomalies, discrepancies, and apparently paradoxical observations obtained by traditional analysis of data from pulse/chase experiments. Including the recycling of cellu...Continue Reading

References

Feb 7, 1998·The Journal of Biological Chemistry·S U Tetzloff, O A Bizzozero
Dec 14, 2002·Trends in Cell Biology·Marie-José Bijlmakers, Mark Marsh
Dec 21, 2002·Pharmacology & Therapeutics·Riad Qanbar, Michel Bouvier

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Citations

Apr 28, 2010·Proceedings of the National Academy of Sciences of the United States of America·Mingzi M ZhangHoward C Hang
Aug 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Daniel KümmelMichael Veit
Nov 17, 2005·Journal of Cellular Biochemistry·Wiesława Leśniak, Jacek Kuźnicki
Aug 30, 2005·Current Opinion in Neurobiology·Kun Huang, Alaa El-Husseini
Feb 5, 2009·Molecular Membrane Biology·Sonia L Planey, David A Zacharias
Apr 6, 2006·The Journal of Biological Chemistry·Ulla E Petäjä-RepoMichel Bouvier

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