Determination of protein concentration in raw milk by mid-infrared fourier transform infrared/attenuated total reflectance spectroscopy

Journal of Dairy Science
Yael EtzionItzhak Shmulevich

Abstract

This study investigates the potential use of attenuated total reflectance spectroscopy in the mid-infrared range for determining protein concentration in raw cow milk. The determination of protein concentration is based on the characteristic absorbance of milk proteins, which includes 2 absorbance bands in the 1500 to 1700 cm(-1) range, known as the amide I and amide II bands, and absorbance in the 1060 to 1100 cm(-1) range, which is associated with phosphate groups covalently bound to casein proteins. To minimize the influence of the strong water band (centered around 1640 cm(-1)) that overlaps with the amide I and amide II bands, an optimized automatic procedure for accurate water subtraction was applied. Following water subtraction, the spectra were analyzed by 3 methods, namely simple band integration, partial least squares (PLS) and neural networks. For the neural network models, the spectra were first decomposed by principal component analysis (PCA), and the neural network inputs were the spectra principal components scores. In addition, the concentrations of 2 constituents expected to interact with the protein (i.e., fat and lactose) were also used as inputs. These approaches were tested with 235 spectra of standardized ...Continue Reading

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Citations

Oct 4, 2012·Animal : an International Journal of Animal Bioscience·M H T Maurice-Van EijndhovenM P L Calus
Nov 3, 2012·Journal of Biomedicine & Biotechnology·Anahita Dehkhoda EckardWilliam Gibbons
Dec 31, 2014·Microbial Cell Factories·Bertrand RaynalPatrick England
Jan 21, 2014·Journal of Dairy Science·M De MarchiM Penasa
Mar 25, 2017·Analytica Chimica Acta·Julia KuligowskiBernhard Lendl
Jul 20, 2012·Biotechnology and Bioengineering·Florian CapitoBernd Stanislawski
Apr 4, 2013·Journal of Dairy Science·B S DagnachewT Adnøy
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Feb 29, 2020·Critical Reviews in Food Science and Nutrition·Zhuozhuo Zhu, Wenchuan Guo
Feb 26, 2020·PeerJ·Isadora Kaline Camelo Pires de Oliveira GaldinoFlávia Carolina Alonso Buriti
May 14, 2020·Journal of Animal Science and Biotechnology·Sadjad Danesh MesgaranBjörn Kuhla
Jul 26, 2017·Journal of Food Science and Technology·Tatiane Barbosa CoitinhoPaulo Fernando Machado
Jan 19, 2021·Journal of Dairy Science·H Toledo-AlvaradoA I Vazquez
May 10, 2007·Journal of Agricultural and Food Chemistry·Fangyu HuMasaru Tanokura
May 1, 2021·Animals : an Open Access Journal From MDPI·Chao DuShujun Zhang
Jan 24, 2013·Journal of Agricultural and Food Chemistry·Poliana M SantosLuis E Rodriguez-Saona

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