PMID: 1201753Jul 1, 1975Paper

Determination of protein-ligand binding constants at equilibrium in biological samples

European Journal of Biochemistry
J P Blondeau, P Robel

Abstract

Protein-ligand complexes can be separated functionally into two classes. "Specific" binding is characterized, in relative terms, by a high affinity for the ligand and a low binding capacity. "Non-specific" binding is characterized by a low affinity and a very large capacity. The calculation of equilibrium binding constants for any specific protein-ligand interaction requires the exact determination of the unbound ligand concentration and the specifically bound ligand concentration. These determinations usually require corrections for the contribution of non-specific binding. The use of two correction terms, kn and f, is proposed: kn is the product of the affinity constant k times the number of binding sites n of the non-specific components, while f is the fraction of the non-specific binding included in the experimental estimates of bound ligand. Several theoretical solutions using these terms are proposed for the calculation of specific binding constants. The practical choice of the correction factor may be different when the simultaneous measurement of the affinity constant and maximum number of binding sites, or when only the latter, is desired. In the case of complex binding systesm containing more than one specific compone...Continue Reading

References

Nov 1, 1970·European Journal of Biochemistry·A Danchin, M Guéron
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Citations

Jan 1, 1988·European Journal of Drug Metabolism and Pharmacokinetics·T AdzetM Merlos
Jan 1, 1990·Journal of Psychiatric Research·B BondyR R Engel
Oct 1, 1980·Journal of Steroid Biochemistry·H Braunsberg, K D Hammond
Feb 1, 1981·Journal of Steroid Biochemistry·E A Thompson
Mar 1, 1979·Molecular and Cellular Endocrinology·A GroyerP Robel
Nov 1, 1979·Molecular and Cellular Endocrinology·C Monet-KuntzA Locatelli
Jun 1, 1980·Molecular and Cellular Endocrinology·G Barbanel, I Assenmacher
Oct 1, 1980·Molecular and Cellular Endocrinology·J L Borgna, H Rochefort
Feb 1, 1980·Molecular and Cellular Endocrinology·E A Thompson
Mar 1, 1983·Molecular and Cellular Endocrinology·C Monet-Kuntz, M Terqui
Sep 15, 1975·European Journal of Biochemistry·M Best-BelpommeE E Baulieu
Jan 1, 1979·The Journal of Pharmacy and Pharmacology·J Romer, M H Bickel
Feb 9, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Jean-Louis Borgna
Jul 1, 1988·European Journal of Cancer & Clinical Oncology·S M Thorpe, C Rose
Jan 28, 2006·The Journal of Steroid Biochemistry and Molecular Biology·Sigrid AliauJean-Louis Borgna
Mar 1, 1980·Analytical Biochemistry·J G NørbyJ Jensen
Nov 1, 1977·European Journal of Biochemistry·G BarbanelJ C Mani
Jan 1, 1981·Journal of Receptor Research·M E ReithA Lajtha
Jan 6, 1984·Science·K E Light

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