Determination of protein synthesis in human rectal cancer in situ by continuous [1-13C]leucine infusion

The American Journal of Physiology
W H HartlF W Schildberg

Abstract

Previous studies on human colorectal tumor protein synthesis in situ relied on techniques that required intra- or perioperative sampling to obtain a sufficient biopsy size. The purpose of the present study was to develop a new technique by use of new mass spectrometry equipment [capillary gas chromatography (GC)-combustion isotope ratio mass spectrometry (IRMS)], which allows reduction of the necessary sampling size. Thereby, tumor sampling could be done via conventional rectosigmoidoscopy, excluding the need for further disturbing invasive measures. Fifteen postabsorptive patients with localized rectal cancer received a primed-constant infusion of [1-13C]leucine (0.16 mumol.kg-1.min-1 constant, 9.6 mumol/kg prime). Forceps biopsies were taken after 3 and 6 h. In five subjects, tumor tissue and normal mucosa were studied simultaneously. Determination of protein-bound leucine enrichment was done by GC-IRMS, and GC-quadrupole MS was used to determine tracer-to-tracee ratios (tracer/tracee) for free intracellular leucine. GC-MS data demonstrated achievement of a steady state in the precursor pool enrichment after 3 h of isotope infusion (tracer/tracee at 3 h: 6.34 +/- 0.46%, at 6 h: 6.58 +/- 0.38%). Calculation of tumor protein sy...Continue Reading

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Citations

Mar 11, 2000·Current Opinion in Clinical Nutrition and Metabolic Care·W Meier-Augenstein
Apr 6, 2000·American Journal of Physiology. Endocrinology and Metabolism·P RittlerW H Hartl
Jan 24, 2003·American Journal of Physiology. Endocrinology and Metabolism·Peter RittlerWolfgang H Hartl
Mar 7, 2006·JPEN. Journal of Parenteral and Enteral Nutrition·Peter RittlerWolfgang H Hartl
Jun 18, 2005·JPEN. Journal of Parenteral and Enteral Nutrition·Peter RittlerWolfgang H Hartl
Jun 23, 1999·Journal of Chromatography. a·W Meier-Augenstein

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