PMID: 3766963Aug 15, 1986Paper

Determination of proteins and sulfobetaine with the Folin-phenol reagent

Analytical Biochemistry
M Ledoux, F Lamy

Abstract

This paper describes a method for the quantitative analysis of solutions containing a mixture of proteins and sulfobetaine. In a preliminary step the proteins, which interfere with the detergent assay, are separated by precipitation with trichloroacetic acid (8%). The insoluble fraction, dissolved in NaOH (1.0 N), and the soluble fraction, containing the detergent, are treated with the Folin-Ciocalteu phenol reagent, essentially following the method of O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall (1951, J. Biol. Chem. 193, 265-275). The absorbance of the protein fraction is read, as usual at 750 nm, while that of the detergent solution is read at 342 nm. At this wavelength, sulfobetaine, treated with the Folin reagent, absorbs strongly, the absorbances being proportional to its concentration up to 1.5 mg/ml.

References

Jun 15, 1978·Analytical Biochemistry·A Gonenne, R Ernst

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Citations

Jun 21, 2002·Biochemical and Biophysical Research Communications·Shohei YamamuraEiichi Tamiya
Oct 31, 2007·Journal of Environmental Sciences (China)·Ya-Peng ChaoShi-Jun Qian
Oct 20, 2005·Journal of Bioscience and Bioengineering·Shohei YamamuraEiichi Tamiya
Sep 24, 2014·Bioresource Technology·Yonglan XiGuangyin Chen
Oct 23, 2012·International Journal of Experimental Pathology·Marcella NebbiosoNicola Pescosolido
May 21, 2009·Journal of Basic Microbiology·Ashraf S A El-Sayed
Oct 17, 2013·Bioresource Technology·Hengfeng MiaoWenquan Ruan
Sep 19, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Laurence Zanna, Jean-François Haeuw
Nov 15, 2016·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Nina DuanLingling Dai
Feb 20, 2019·Journal of the Science of Food and Agriculture·Hao HuangGui-Tang Chen
Jun 25, 2019·Water Environment Research : a Research Publication of the Water Environment Federation·Jia HeHongbin Zhou
Oct 10, 2006·Journal of Bioscience and Bioengineering·Yasunori SuzukiKunihiko Watanabe

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