Determination of proteins at nanogram levels with Bordeaux red based on the enhancement of resonance light scattering

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Suling FengJing Fan

Abstract

A simple, sensitive and selective method was proposed for the determination of proteins by using a resonance light scattering technique. The weak resonance light scattering (RLS) of Bordeaux red (BR) can be enhanced greatly in the pH range 3.87-3.96 by the addition of micro amounts of proteins, resulting in four characteristic peaks in the wavelength range 250-600 nm. At the maximal wavelength of 363 nm, the enhanced RLS is proportional to the concentration of proteins in the range 0.12-10.8 microg ml-1 for bovine serum albumin (BSA) and 0.24-18.0 microg ml-1 for human serum albumin (HSA). The detection limits were 40.0 and 52.9 ng ml-1 for BSA and HSA, respectively. The present method has been applied to the determination of total proteins in human serum, urine and saliva samples. The obtained results are in good agreement with those obtained by the Bradford method with relative standard deviations (R.S.D.) of 0.9-2.3%.

References

Jan 1, 1971·Clinica Chimica Acta; International Journal of Clinical Chemistry·B T DoumasH G Biggs
Oct 14, 2004·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Zheng Ping LiFeng Chi Wang
Oct 21, 2004·Analytical Biochemistry·Guang-Chao ZhaoZhou-Sheng Yang

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Citations

Jan 15, 2010·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Changqun Cai, Xiaoming Chen
May 3, 2008·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Ning-Li Tang, Jin-Yun Peng
Sep 18, 2007·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Zhan Guang ChenYa Li Han
May 29, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Ai-Hui LiangZhi-Liang Jiang
Jul 28, 2006·Luminescence : the Journal of Biological and Chemical Luminescence·Aiyun LiLinpei Jin
Apr 7, 2009·Luminescence : the Journal of Biological and Chemical Luminescence·Juan LiYihua Zhang
Sep 29, 2011·Luminescence : the Journal of Biological and Chemical Luminescence·Yanru YunJianhua Jin
May 15, 2007·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yun Fu Ou-YangYing Wang

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