PMID: 12766551May 27, 2003Paper

Determination of pyronaridine in whole blood by automated solid phase extraction and high-performance liquid chromatography

Therapeutic Drug Monitoring
Daniel BlessbornY Bergqvist

Abstract

A new extraction procedure for the analysis of pyronaridine in whole blood is presented. A weak cation exchanger with a carboxylic acid (CBA) sorbent was found to be a suitable solid phase sorbent for the extraction of pyronaridine. High-performance liquid chromatography with UV detection at 278 nm and an electrochemical detector at +0.75 V is used. The electrochemical detector gives higher selectivity than the UV detector. The separation was performed using a C18 reversed phase column with mobile phase of acetonitrile-phosphate buffer (0.01 mol/L, pH 2.5)- sodium perchlorate (1.0 mol/L; 22:77:1, v/v/v). The within-day RSDs were below 5% at all concentration levels between 75 nmol/L and 1500 nmol/L, and the between-day RSDs were below 14% at all concentration levels. The limit of quantification was about 50 nmol/L in 1000 microL whole blood with an RSD of 20% or less on a day-to-day basis. The stability of pyronaridine is increased if the pH is less than 3 in water solutions. In whole blood, the concentration decreases by about 10% for each freeze-thaw cycle performed. At room temperature (about 22 degrees C), pyronaridine concentration in whole blood decreases by about 10% within 12 to 24 hours.

References

Jan 1, 1992·Transactions of the Royal Society of Tropical Medicine and Hygiene·C ChangC Jantanavivat
Feb 1, 1995·Journal of Pharmaceutical and Biomedical Analysis·D DadgarJ W Hooper
Feb 1, 1996·The American Journal of Tropical Medicine and Hygiene·S LooareesuwanW H Wernsdorfer
Feb 7, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·R Causon
Mar 7, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·S D JayaramanV Navaratnam
Nov 1, 1991·Parasitology Today·S Fu, S H Xiao

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Citations

Apr 10, 2010·Journal of Biomedicine & Biotechnology·Sang Hyun Park, Kannampalli Pradeep
Dec 17, 2015·Antimicrobial Agents and Chemotherapy·Amal AyyoubLawrence Fleckenstein

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