Determination of rat liver triglycerides by gas-liquid chromatography and reversed-phase high-performance liquid chromatography

Journal of Chromatography. B, Biomedical Sciences and Applications
J S PeronaV Ruiz-Gutiérrez

Abstract

Rats fed with a fat-free or an olive oil-rich diet were employed to compare the response of two chromatographic techniques in the determination of rat liver triglyceride (TG) molecular species composition. Gas-liquid chromatography (GLC) on polarizable liquid phase and reversed-phase high-performance liquid chromatography (RP-HPLC) have been commonly employed for TG analysis, obtaining a similar number of chromatographic peaks when used for animal tissue TG determination. In the present study similar results were achieved with regard to most relevant chromatographic peaks, however, important differences were found in the content of minor TGs. Indeed, RP-HPLC permitted separation of long chain polyunsaturated fatty acids, which were not detected by GLC, while the latter technique reported a higher number of myristoyl-containing TG species. RP-HPLC analysis reported a greater number of TGs, with more similarity to a random composition, made up from the liver fatty acid composition. Therefore, it was concluded that utilization of both techniques would be helpful for liver TG analysis as the use of only one of them does not provide a complete profile of liver TGs. Nevertheless RP-HPLC seems to be more useful for this purpose since ...Continue Reading

References

Aug 1, 1992·The American Journal of Physiology·X L Chen, C M Rembold
Oct 23, 1992·Journal of Chromatography·V Ruiz-GutierrezJ Villar
Mar 13, 1992·Journal of Chromatography·V Ruiz-GutierrezJ J Rios
Jun 1, 1984·Canadian Journal of Biochemistry and Cell Biology = Revue Canadienne De Biochimie Et Biologie Cellulaire·J J Myher, A Kuksis
Jan 1, 1984·The American Journal of Physiology·R L CurtisJ L Madara
Dec 22, 1993·Journal of Chromatography·V Ruíz-GutiérrezF Pérez-Jiménez
Jul 1, 1993·Journal of Dairy Science·J GrestiJ Bezard
Sep 15, 1995·Journal of Chromatography. B, Biomedical Applications·V Ruiz-Gutiérrez, L J Barron

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Citations

Jul 7, 2011·Analytical and Bioanalytical Chemistry·Luciane C AlbericiRosana M Alberici
Oct 18, 2011·Journal of Separation Science·Christian HellmuthBerthold Koletzko
Nov 26, 1999·The Journal of Nutrition·R AbiaV Ruiz-Gutiérrez
Nov 21, 2001·Journal of Agricultural and Food Chemistry·Y M PachecoF J Muriana

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