Determination of rate constants of the reactions of thiols with superoxide radical by electron paramagnetic resonance: critical remarks on spectrophotometric approaches

Archives of Biochemistry and Biophysics
S I DikalovG Zimmer

Abstract

Two new EPR approaches were developed for determination of rate constants of reaction glutathione (GSH), N-(2-mercaptopropionyl) glycine (MPG), dihydrolipoic acid (BNL), and tetranor-dihydrolipoic acid (TNL) with superoxide radical. In both cases the competition between thiols and spin-trap 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) for superoxide radical was used. In the first method the dependence of amplitude of EPR spectrum of DMPO-OOH spin adduct on concentration of thiols in a superoxide-generating system was studied. In the second approach the changes in reduced thiol concentration due to reaction with superoxide radical were measured by nitroxide biradical containing disulfide bond. Observed rate constants were the following: GSH, 1.8 x 10(5) M-1s-1; MPG, 2.2 x 10(5) M-1s-1; TNL, 1.2 x 10(5) M-1s-1; BNL, 2.5 x 10(5) M-1s-1; DHL, 4.8 x 10(5) M-1s-1. The determination of the rate constants of reaction of superoxide radical with thiols by spectrophotometrical cytochrome C assay could result in an underestimation of the values due to the reduction of cytochrome C by thiols. Use of epinephrine for this purpose could lead to an overestimation of experimental rate constants because the adrenochrome formed in the reaction of epine...Continue Reading

Citations

Apr 14, 2009·Antioxidants & Redox Signaling·Derick HanNeil Kaplowitz
Mar 25, 2000·Journal of Molecular and Cellular Cardiology·M N SharikabadO Brørs
Sep 1, 1997·General Pharmacology·G P BiewengaA Bast
Sep 4, 2015·Free Radical Research·Madia TrujilloRafael Radi
Sep 2, 2008·Journal of Photochemistry and Photobiology. B, Biology·Lyudmila V ChekulayevaIgor N Shevchuk
Apr 10, 1999·Experimental Gerontology·C Schöneich
Oct 21, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Robert M BellMichael J Shattock
Jun 19, 2019·Alcoholism, Clinical and Experimental Research·Pablo BaliñoMaría Muriach
Feb 6, 2020·Pharmaceutical Research·Christian Schöneich
Mar 15, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Hong Yan ZhangZhenhai Yao
Dec 7, 2010·Free Radical Research·Sergey I DikalovIgor A Grigor'ev
Feb 17, 2001·American Journal of Physiology. Heart and Circulatory Physiology·Z YaoP T Schumacker
Apr 1, 2009·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Bruno Cardey, Mironel Enescu
Apr 6, 2019·Nature Communications·Zhen WangDacheng Wei
Jun 9, 1999·Mechanisms of Ageing and Development·C SchöneichD J Bigelow
Sep 1, 1999·Free Radical Biology & Medicine·C C Winterbourn, D Metodiewa
Nov 14, 1997·Journal of Biochemical and Biophysical Methods·V V KhramtsovG Zimmer
Apr 18, 2002·Free Radical Biology & Medicine·A AgbasE K Michaelis
Feb 15, 2001·Journal of Pharmaceutical Sciences·S Hovorka, C Schöneich
Aug 28, 2018·Chemical Reviews·Saba ParvezYimon Aye
Oct 3, 2002·Journal of the American Chemical Society·Roman BoulatovChristopher J Sunderland

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.