Determination of residual trifluoroacetate in protein purification buffers and peptide preparations by ion chromatography

Journal of Chromatography. a
E Kaiser, Jeff S Rohrer

Abstract

Trifluoroacetate (TFA) is commonly used in a variety of pharmaceutical applications. Because of its toxic nature, it is important to reliably measure the effective removal of TFA. We developed an ion chromatography (IC) method to determine the concentration of residual TFA in samples found in the pharmaceutical industry. A high-capacity anion-exchange column was used to separate trace trifluoroacetate from an excess of chloride, phosphate, and other anions without the need for sample preparation. TFA was detected by suppressed conductivity. A method with four KOH eluent step changes was optimized and reproducibly executed using automated generation of the KOH eluent. We used this method to determine TFA in the following samples: a phosphate-buffered saline (PBS), an acetate-buffered saline containing protein, and a commercial peptide. The method detection limits for TFA in these samples were all less than 90 ng/ml.

Citations

Sep 15, 2009·Journal of Peptide Science : an Official Publication of the European Peptide Society·Valentijn VergoteBart De Spiegeleer
Jan 19, 2012·Journal of Peptide Science : an Official Publication of the European Peptide Society·Wojciech MrozikWojciech Kamysz
Dec 17, 2014·Biochimica Et Biophysica Acta·Julia MisiewiczAnne S Ulrich
May 4, 2018·International Journal of Peptide Research and Therapeutics·Karol SikoraWojciech Kamysz
Dec 9, 2020·Pharmaceuticals·Karol SikoraWojciech Kamysz
Mar 12, 2021·Advanced Drug Delivery Reviews·Yajie ZhangRobert O Williams
Jun 15, 2005·Analytical Chemistry·R K Gilpin, L A Pachla
Jan 18, 2011·Analytical Chemistry·Joana DiekmannJulie L Herberg

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