Determination of rimonabant in human plasma and hair by liquid chromatography-mass spectrometry

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Stanislas Grassin DelyleJean Claude Alvarez

Abstract

Rimonabant is the first therapeutically relevant cannabinoid antagonist, licensed in Europe for treatment of obesity when a risk factor is associated. The objective of this study was to develop and validate a method for measurement of rimonabant in human plasma and hair using liquid chromatography coupled to mass spectrometry (LC-MS/MS). Rimonabant and AM-251 (internal standard) were extracted from 50 microL of plasma or 10 mg of hair using diethylether. Chromatography was performed on a 150 mm x 2.1 mm C18 column using a mobile phase constituted of formate buffer/acetonitrile. Rimonabant was ionized by electrospray in positive mode, followed by detection with mass spectrometry. Data were collected either in full-scan MS or in full-scan MS/MS mode, selecting the ion m/z 463.1 for rimonabant and m/z 555.1 for IS. The most intense product ion of rimonabant (m/z 380.9) and IS (m/z 472.8) were used for quantification. Calibration curves covered a range from 2.5 (lower limit of quantification) to 1000.0 ng/mL (upper limit of quantification) in plasma and from 2.5 to 1000.0 pg/mg in hair. Validation results demonstrated that rimonabant could be accurately and precisely quantified in both matrixes: accuracy and precision were within 8...Continue Reading

References

Sep 30, 2005·Nature·Meredith Wadman
Feb 16, 2006·JAMA : the Journal of the American Medical Association·F Xavier Pi-SunyerUNKNOWN RIO-North America Study Group
May 26, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Magali Gary-BoboMohammed Bensaid

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