Determination of salbutamol enantiomers in human plasma and urine by chiral high-performance liquid chromatography

Journal of Chromatography. B, Biomedical Applications
D W Boulton, J P Fawcett

Abstract

Enantiomers of salbutamol were directly separated (Rs = 1.16) and quantitated at therapeutic concentrations after solid-phase extraction from human plasma and urine by normal-phase high-performance liquid chromatography on a chiral column with fluorescence detection. The assay was linear for each enantiomer between 1.25 and 500 ng ml-1 and had a minimum limit of detection of 250 pg ml-1. A 3-ml plasma or 1-ml urine sample was required for quantitation at therapeutic doses. Inter-day variation was 5.0% for S-(+)- and 6.5% for R-(-)-salbutamol. The assay was used to compare enantioselective disposition after single doses of racemate by the intravenous, oral and rectal routes.

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Citations

Jan 1, 1996·Clinical Reviews in Allergy & Immunology·D W Boulton, J P Fawcett
Jan 25, 2003·Journal of Biochemical and Biophysical Methods·Antonio Marzo, Erich Heftmann
Jan 1, 1996·British Journal of Clinical Pharmacology·D W Boulton, J P Fawcett
Oct 6, 1999·American Journal of Respiratory and Critical Care Medicine·R DhandM J Tobin
Oct 25, 2006·American Journal of Respiratory and Critical Care Medicine·Bill T Ameredes, William J Calhoun
May 16, 2002·Pharmacotherapy·Michael J AsmusRichard C Ahrens
Mar 10, 2001·Clinical Pharmacokinetics·D W Boulton, J P Fawcett
Jan 1, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Zhenzhen WangWilly R G Baeyens
Sep 6, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ting ZhouWen Tan
Dec 7, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Baomi LiuYuru Geng
Jan 22, 2002·Pediatric Pulmonology·D SlatteryA A Colin
Mar 29, 2002·The Annals of Pharmacotherapy·Majid VakilyDion Brocks
Jan 15, 2004·American Journal of Respiratory Medicine : Drugs, Devices, and Other Interventions·David W Boulton, J Paul Fawcett
Jun 28, 2000·Journal of Biochemical and Biophysical Methods·J L BernalE M Nieto

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