Abstract
A new analytical method, based on liquid chromatography with coulometric detection, has been developed and applied to the determination of selected phenothiazines (chlorpromazine, promazine, fluphenazine and levomepromazine) in human plasma. The drugs were separated on a Discovery pentafluorophenylpropyl column, using a mobile phase composed of acetonitrile (32%) and a pH 1.9 phosphate buffer (68%). Promethazine was used as the internal standard. Detection was carried out at an oxidation potential of +0.500 V. A novel clean-up procedure was developed by means of solid-phase extraction, using cyanopropyl cartridges, which gave good extraction yield for all the analytes, with absolute recovery values higher than 91.0%. The detector response was linear over a plasma concentration range of 0.5-250.0 ng mL(-1) for chlorpromazine, promazine and levomepromazine and of 0.2-4.0 ng mL(-1) for fluphenazine. Precision results, expressed by the intra-day and the inter-day relative standard deviation values, were good, being lower than 3.9%. Accuracy data were satisfactory as well. The method has been successfully applied to the analysis of drug plasma levels of psychiatric patients undergoing therapy with selected phenothiazines.
References
Sep 1, 1990·The Analyst·P KintzP Mangin
Jul 1, 1994·Journal of Chromatography. B, Biomedical Applications·M BagliG Höflich
Nov 24, 1999·Rapid Communications in Mass Spectrometry : RCM·H SenoO Suzuki
Sep 28, 2000·Journal of Mass Spectrometry : JMS·T KumazawaO Suzuki
Oct 24, 2001·Forensic Science International : Synergy·C L WinekT W Balzer
Jan 30, 2002·Journal of Pharmaceutical and Biomedical Analysis·M M Hefnawy
Feb 26, 2003·Drugs·A Cario AltamuraEmanuela Mundo
Jul 30, 2005·BMJ : British Medical Journal·Sean C Sweetman
Oct 19, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Laura MercoliniMaria Augusta Raggi
Citations
Dec 17, 2009·Naunyn-Schmiedeberg's Archives of Pharmacology·Britta Haenisch, Heinz Bönisch
Apr 21, 2011·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Jing ChenZou Su
Feb 22, 2012·Sensors·Ahmed Khudhair HassanSuad Mustafa Al-Araji
Jul 24, 2012·Talanta·P Halaburda, J V García Mateo
Mar 25, 2011·Journal of Chromatography. a·Takeshi KumazawaKeizo Sato
Oct 22, 2009·Rapid Communications in Mass Spectrometry : RCM·Markus Persike, Michael Karas
Jul 6, 2010·Journal of the Science of Food and Agriculture·Wei LiuRimo Xi
Oct 12, 2013·Journal of Separation Science·Homeira EbrahimzadehFatemeh Mirbabaei
Jul 13, 2013·Analytica Chimica Acta·Li YaoXiaolan Zhu
Apr 27, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·JoEtta M JuenkeGwendolyn A McMillin
Mar 19, 2014·Analytical Biochemistry·Bao Long GaoJian Ping Wang
Jun 30, 2016·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Fang S ShiJian P Wang
Feb 19, 2019·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kanae Nishimura, Jun Haginaka
Jul 7, 2018·Journal of Food and Drug Analysis·Wei-Bin TsengSzu-Hua Chen
Mar 26, 2021·International Journal of Analytical Chemistry·Wei WangJuan Shen
Apr 16, 2021·Biomedical Chromatography : BMC·Liju FanZhanjun Dong