Determination of selected stimulants in urine for sports drug analysis by solid phase extraction via cation exchange and means of liquid chromatography-tandem mass spectrometry

European Journal of Mass Spectrometry
Andreas ThomasMario Thevis

Abstract

Stimulatory substances applied during competition possess a reasonable potential as performance enhancing agents and their misuse in elite sport has been frequently reported during the last few decades. An analytical method for the qualitative determination of selected stimulants containing a primary or secondary amine moiety in human urine for doping control purposes was developed. A rapid and highly specific procedure based on a sample preparation using weak cation exchange solid phase extraction (SPE-XCW) followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) with a C6-Phenyl analytical column allowed the unambiguous identification of the target analytes down to low ng mL(-1) concentration levels. Validation provided recovery rates of better than 75%, precisions of less than 20% and a linear approximation in the required working range (10-750 ng mL(-1)) were obtained for 19 different target compounds. This method provides a rugged and highly specific alternative to the established method utilising gas or liquid chromatography after liquid-liquid extraction.

References

Oct 1, 1976·British Journal of Sports Medicine·M Donike
Dec 6, 1996·Journal of Chromatography. B, Biomedical Applications·P Hemmersbach, R de la Torre
Dec 9, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·P MarquetG Lachâtre
Jul 2, 2004·Therapeutic Drug Monitoring·Rafael de la TorreJordi Camí
Feb 14, 2006·Rapid Communications in Mass Spectrometry : RCM·K DeventerF T Delbeke
Jun 27, 2006·British Journal of Sports Medicine·L AvoisM Saugy
Aug 5, 2006·Mass Spectrometry Reviews·Mario Thevis, Wilhelm Schänzer
Aug 24, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·W Van ThuyneF T Delbeke

❮ Previous
Next ❯

Citations

Apr 29, 2009·Briefings in Functional Genomics & Proteomics·Kelli G KlineChristine C Wu
Mar 23, 2011·Analytical and Bioanalytical Chemistry·Mario ThevisWilhelm Schänzer
Sep 21, 2011·Biomedical Chromatography : BMC·Mario ThevisWilhelm Schänzer
Mar 3, 2015·Drug Testing and Analysis·Núria MonfortRosa Ventura
Sep 22, 2020·Analytical and Bioanalytical Chemistry·Claudia BressanRosa Ventura
Apr 12, 2011·Analytical and Bioanalytical Chemistry·K DeventerP Van Eenoo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.