Determination of serum bone-specific alkaline phosphatase isoenzyme activity in captive Asian elephants (Elephas maximus) using an agarose gel electrophoresis method

The Journal of Veterinary Medical Science
Kazuya TakehanaNorio Yamagishi

Abstract

The bone-specific alkaline phosphatase (ALP) isoenzyme activity was measured in 51 serum samples from four captive Asian elephants (Elephas maximus) using a conventional method with wheat germ lectin precipitation and a commercial agarose gel electrophoresis (AGE) kit; the isoenzymes were designated as bone-specific ALP (BAP) and ALP isoenzyme 3 (ALP3), respectively. This study examined the suitability of the AGE kit for analyzing blood biochemistry in Asian elephants. The serum ALP3 and BAP activities were strongly positively correlated and met the evaluation criteria for agreement using Bland-Altman analysis. The results indicate that the AGE kit can be used to examine the blood biochemistry in Asian elephants instead of the conventional method.

References

Aug 1, 1988·American Journal of Clinical Pathology·W E Schreiber, L C Sadro
Jan 1, 1969·The British Journal of Ophthalmology·I S Begg
Mar 1, 1993·The Journal of Bone and Joint Surgery. British Volume·K S LeungK M Lee
Dec 29, 2000·Veterinary Radiology & Ultrasound : the Official Journal of the American College of Veterinary Radiology and the International Veterinary Radiology Association·K M Hittmair, H D Vielgrader
Feb 21, 2002·The Journal of Veterinary Medical Science·Hiroshi ItohKatsuaki Takase
Feb 13, 2003·Journal of Clinical Monitoring and Computing·L A Critchley, J A Critchley
Sep 11, 2003·Veterinary Clinical Pathology·M J Allen
Nov 7, 2003·The Journal of Clinical Endocrinology and Metabolism·Berthold FohrMarkus J Seibel
May 18, 2007·Journal of Clinical Laboratory Analysis·Kinue OoiTsutomu Nobori
Jul 7, 2007·Journal of Biopharmaceutical Statistics·J Martin Bland, Douglas G Altman
Dec 30, 2008·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Conor KilgallonBrendan Jackson
Mar 5, 2011·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Carola KaulfersAndreas Knieriem
Apr 7, 2011·The Journal of Toxicological Sciences·Kazuhisa HatayamaYoshinaka Urasoko
Nov 13, 2012·Journal of Bone and Mineral Metabolism·Yoshiki NishizawaNoriko Yoshimura
Jan 9, 2014·Annals of Clinical Biochemistry·Thinn Thinn Hlaing, Juliet E Compston
Nov 21, 2017·The Journal of Veterinary Medical Science·Kazuya TakehanaNorio Yamagishi

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BETA
electrophoresis

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Edbank

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