Determination of serum levels of unesterified lathosterol by isotope dilution-mass spectrometry

Scandinavian Journal of Clinical and Laboratory Investigation
E LundI Björkhem


The synthesis of 2H3-labelled lathosterol is described. This compound was used together with 2H7-labelled cholesterol for simultaneous assay of unesterified lathosterol and cholesterol in serum by isotope dilution-mass spectrometry. After addition of a fixed amount of the two internal standards to a fixed amount of serum (in general 25 microliter), the steroids were extracted with chloroform and subjected to Lipidex 5000 chromatography. The fraction containing cholesterol and lathosterol was converted into trimethylsilyl ether and subjected to mass spectrometric analysis with selected monitoring of the ions at m/z 458 (molecular ion of the trimethylsilyl ether derivative of unlabelled cholesterol and lathosterol), m/z 461 (molecular ion of derivative of 2H3-labelled lathosterol) and m/z 465 (molecular ion of derivative of 2H7-labelled cholesterol). Individual standard curves were used for assay of each steroid. Under the conditions employed, the coefficient of variation of the two assays was less than 6%. In different recovery experiments the maximal difference between expected and found values was less than 7%. Using a less accurate method for analysis of lathosterol, we have shown previously that there is a high correlation b...Continue Reading


Aug 1, 1979·CRC Critical Reviews in Clinical Laboratory Sciences·I Björkhem
Jul 31, 1974·Clinica Chimica Acta; International Journal of Clinical Chemistry·I BjörkhemL Svensson
Jun 1, 1963·Analytical Biochemistry·M MAKITA, W W WELLS
Jan 1, 1947·Helvetica Chimica Acta·W BUSER

❮ Previous
Next ❯


Mar 21, 1998·Lipids·A al-ShurbajiL Berglund
Aug 3, 2002·European Journal of Clinical Investigation·C-G HillebrantC Einarsson
Jul 26, 1990·The New England Journal of Medicine·E ReihnérB Angelin
Jul 24, 2012·The Journal of Clinical Investigation·Saida OmarovaIrina A Pikuleva
Jul 28, 2009·Journal of Lipid Research·Dragana D BojanicPeter A Edwards
Apr 4, 2008·International Review of Psychiatry·Alka Aneja, Elaine Tierney
Apr 24, 2013·Journal of Internal Medicine·E EkholmC Pramfalk
Dec 17, 2008·Biochemical and Biophysical Research Communications·Zhao-Yan JiangGösta Eggertsen
Jul 5, 2005·Biochimica Et Biophysica Acta·Charlotte MurphyMats Gåfvels
Jul 30, 2014·The American Journal of Pathology·Aicha SaadaneIrina A Pikuleva
Jan 10, 2012·Biochemical and Biophysical Research Communications·Cristine SkogastiernaLinda Björkhem-Bergman
Sep 9, 2008·Biochemical and Biophysical Research Communications·Jure AcimovicDamjana Rozman
Oct 26, 2007·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Lorenzo ArnaboldiAlberto Corsini
Aug 31, 2014·Journal of Lipid Research·Ylva BondeMats Rudling
Oct 9, 2014·Journal of Lipid Research·Wenchao ZhengIrina A Pikuleva
Dec 6, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Suzanne LindBo Angelin
Jul 29, 2006·American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics : the Official Publication of the International Society of Psychiatric Genetics·Elaine TierneyRichard I Kelley
Oct 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S KurokiM Tanaka
Apr 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D StåhlbergB Angelin
Apr 28, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Juan CastroFlavio Nervi
Oct 9, 2009·Scandinavian Journal of Gastroenterology·Kjell-Arne UngAntal Bajor
Dec 20, 2013·The Journal of Biological Chemistry·Natalia MastIrina A Pikuleva
Nov 1, 1990·Scandinavian Journal of Clinical and Laboratory Investigation·E LundI Björkhem
Sep 29, 2015·The Journal of Clinical Endocrinology and Metabolism·Camilla PramfalkLeanne Hodson
Aug 2, 2019·The Journal of Clinical Endocrinology and Metabolism·Fredrik RosqvistUlf Risérus

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Landau-Kleffner Syndrome

Landau Kleffner syndrome (LKS), also called infantile acquired aphasia, acquired epileptic aphasia, or aphasia with convulsive disorder, is a rare childhood neurological syndrome characterized by the sudden or gradual development of aphasia (the inability to understand or express language) and an abnormal electroencephalogram. Discover the latest research on LKS here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.