Determination of seven organosulfur compounds in garlic by high-performance liquid chromatography

Journal of Agricultural and Food Chemistry
Makoto IchikawaKazuhisa Ono

Abstract

The properties of garlic (Allium sativum L.) are attributed to organosulfur compounds. Although these compounds change during cultivation and storage, there is no report of their simultaneous analysis. Here, a newly developed analytical method with a rapid and simple sample preparation to determine four sulfoxides and three gamma-glutamyl peptides in garlic is reported. All garlic samples were simply extracted with 90% methanol solution containing 0.01 N hydrochloric acid and prepared for analysis. Alliin, isoalliin, methiin, cycloalliin, and gamma-l-glutamyl-S-methyl-l-cysteine were determined by normal-phase HPLC using an aminopropyl-bonded column. gamma-l-Glutamyl-S-(2-propenyl)-l-cysteine and gamma-l-glutamyl-S-(trans-1-propenyl)-l-cysteine were separated on an octadecylsilane column. The overall recoveries were 97.1-102.3%, and the relative standard deviation values of intra- and interday precision were lower than 2.6 and 4.6%, respectively. This newly developed method offers some advantages over the currently accepted techniques including specificity, speed, and ease of use and would be useful for chemical and biological studies of garlic and its preparations.

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Citations

Feb 19, 2013·Analytical Biochemistry·Jenny LeeBao-Shiang Lee
Jan 27, 2015·Frontiers in Plant Science·Naoko YoshimotoKazuki Saito
Apr 1, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yukihioro KoderaToshiaki Matsutomo
Jul 9, 2016·Preventive Nutrition and Food Science·Hyun Jung LeeHyeon-Son Choi
Jan 10, 2012·Journal of the Science of Food and Agriculture·Guo LiangZhenjia Zheng
Jun 23, 2019·Journal of the Science of Food and Agriculture·Andrés F Cubillos BojacáMartha P Tarazona-Díaz
Jul 28, 2015·Journal of the Science of Food and Agriculture·Xinyan ZhangXuguang Qiao
Mar 1, 2018·Bioscience, Biotechnology, and Biochemistry·Yusuke AminoYutaka Ishiwatari
Sep 9, 2015·The Plant Journal : for Cell and Molecular Biology·Naoko YoshimotoKazuki Saito
Jul 5, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tharsini SivapalanShikha Saha
May 13, 2019·Environmental Science and Pollution Research International·Cecilia Nwadiuto AmadiOrish Ebere Orisakwe
Nov 19, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wamidh H TalibAsma Ismail Mahmod
May 25, 2021·Critical Reviews in Food Science and Nutrition·Jieying LiAlireza Abbaspourrad
Oct 22, 2020·Journal of Agricultural and Food Chemistry·Pingxiang LiuJing Qiu
Nov 16, 2007·Journal of Agricultural and Food Chemistry·Miguel Aguilar, Francisco Rincón

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