Determination of some pyridinium aldoxime compounds by means of ion-pair reversed-phase high-performance liquid chromatography: application in biological material

Journal of Chromatography
H P BenschopD A Ligtenstein

Abstract

Two reversed-phase high-performance liquid chromatographic systems are presented for the separation and assay of the pyridinium aldoximes benzyl-P2A, HI-6 and obidoxime in aqueous solutions and biological samples. The systems involve a 5-micrometer C18 silica gel stationary phase. The eluent consists of methanol, acetic acid buffer (pH 4.80), a counter ion (per-chlorate or n-octanesulphonate) and a surfactant. The compounds were detected spectrophotometrically at 304 nm. In the concentration range used, linear plots of concentration versus extinction were obtained, both in blood and in water. Detection limits plots of concentration versus extinction were obtained, both in blood and in water. Detection limits, even in blood are satisfactory (0.5-1 microM). Evidence of presented that, at least for HI-6, the addition of counter ions to the system does not lead to the formation of ion pairs to be retained by partition, but rather to a mechanism based on adsorption chromatography.

Citations

Apr 1, 1988·Archives of Toxicology·K SchoeneM Krügel
Sep 5, 2007·Analytical and Bioanalytical Chemistry·B BenkoK Tekes
May 4, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·C GrasshoffL Szinicz
Apr 1, 1996·Pharmacology & Toxicology·B MilicM Nedelijkovic
Jan 13, 2010·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Jana Zdarova KarasovaKamil Kuca
Mar 1, 1983·British Journal of Pharmacology·H P van HeldenO L Wolthuis

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