Determination of stability constants of valinomycin complexes with ammonium and alkali metal ions by capillary affinity electrophoresis

Electrophoresis
Sille EhalaEmanuel Makrlík

Abstract

Capillary affinity electrophoresis (CAE) has been employed to investigate quantitatively the interactions of valinomycin, macrocyclic depsipeptide antibiotic ionophore, with univalent cations, ammonium and alkali metal ions, K(+), Cs(+), Na(+), and Li(+), in methanol. The study involved measuring the change in effective electrophoretic mobility of valinomycin while the cation concentrations in the BGE were increased. The corresponding apparent stability (binding) constants of the valinomycin-univalent cation complexes were obtained from the dependence of valinomycin effective mobility on the cation concentration in BGE using a nonlinear regression analysis. The calculated apparent stability constants of the above-mentioned complexes show the substantially higher selectivity of valinomycin for K(+) and Cs(+) ions over Li(+), Na(+), and NH(4)(+) ions. CAE proved to be a suitable method for the investigation of both weak and strong interactions of valinomycin with small ions.

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Citations

May 27, 2010·Beilstein Journal of Organic Chemistry·Andreas Späth, Burkhard König
Jun 12, 2009·Electrophoresis·Niels H H Heegaard
Dec 2, 2009·Electrophoresis·Václav Kasicka
Dec 8, 2009·Electrophoresis·Andrei R Timerbaev
Aug 8, 2015·Journal of Separation Science·Hassan A AlhazmiHermann Wätzig
May 20, 2015·Journal of Separation Science·Sille Štěpánová, Václav Kašička
May 28, 2014·Journal of Chromatography. a·Martin RůžičkaVáclav Kašička
Nov 1, 2011·Biosensors & Bioelectronics·Ko-Shing ChangYit-Tsong Chen
Jan 16, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xiaoying XuLihong Liu
May 26, 2010·Analytical Chemistry·Nicholas W FrostMichael T Bowser

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