Determination of substrate preference in phosphatidylserine decarboxylation by liquid chromatography-electrospray ionization mass spectrometry

Analytical Biochemistry
Jillonne H Kevala, H Y Kim

Abstract

A method has been developed to determine the substrate preference in phosphatidylserine decarboxylation (PSD), the process by which phosphatidylserine is converted to phosphatidylethanolamine (PE) in the mitochondria. The in vitro assay utilized liposomes containing deuterium-labeled PS molecular species incubated with liver and brain cortex mitochondria, and the conversion of PS to the corresponding PE species was monitored by electrospray ionization mass spectrometry in conjunction with reversed-phase liquid chromatography. Employing this approach we were able to establish for the first time that there exists a substrate preference in PSD in liver (18:0,18:1 > or = 18:0,22:6 > 18:0,20:4-PS) and brain cortex (18:0,22:6 > 18:0,18:1 > 18:0,20:4-PS). The observed PSD molecular species preference, however, did not reflect the mitochondrial PE profile, suggesting that selectivity in other processes such as de novo PE synthesis, intracellular transport of phospholipid molecules, or remodeling by deacylation-reacylation may be important contributors in maintaining a specific lipid profile in mitochondria.

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Citations

Aug 12, 2009·The Journal of Biological Chemistry·Roberta LeonardiSuzanne Jackowski
May 19, 2009·Journal of the American Society for Mass Spectrometry·Bill X Huang, Hee-Yong Kim
Oct 23, 2002·Journal of Neuroscience Research·Gabriela A SalvadorNorma M Giusto
Jan 24, 2009·IUBMB Life·Irmgard Schuiki, Günther Daum
Aug 9, 2011·Biochimica Et Biophysica Acta·Richard M Epand
Nov 20, 2012·Biochimica Et Biophysica Acta·Ville KainuPentti Somerharju
Jul 6, 2014·Progress in Lipid Research·Hee-Yong KimArthur A Spector
Aug 28, 2001·Journal of Mass Spectrometry : JMS
Mar 28, 2007·Journal of Neuroscience Research·Zhiming Wen, Hee-Yong Kim
May 10, 2007·The Journal of Biological Chemistry·Hee-Yong Kim
May 10, 2019·Frontiers in Neuroscience·Angel GaudiosoJose Antonio Rodriguez-Navarro
Mar 12, 2021·Fish Physiology and Biochemistry·Jianshuang LiXin Qi
Jan 7, 2018·Molecular Aspects of Medicine·R J Scott LacombeRichard P Bazinet

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