Determination of subunit composition of Clostridium cellulovorans cellulosomes that degrade plant cell walls.

Applied and Environmental Microbiology
Koichiro MurashimaRoy H Doi

Abstract

Clostridium cellulovorans produces a cellulase enzyme complex (cellulosome). In this study, we isolated two plant cell wall-degrading cellulosomal fractions from culture supernatant of C. cellulovorans and determined their subunit compositions and enzymatic activities. One of the cellulosomal fractions showed fourfold-higher plant cell wall-degrading activity than the other. Both cellulosomal fractions contained the same nine subunits (the scaffolding protein CbpA, endoglucanases EngE and EngK, cellobiohydrolase ExgS, xylanase XynA, mannanase ManA, and three unknown proteins), although the relative amounts of the subunits differed. Since only cellobiose was released from plant cell walls by the cellulosomal fractions, cellobiohydrolases were considered to be key enzymes for plant cell wall degradation.

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Citations

Jun 16, 2004·Nature Reviews. Microbiology·Roy H Doi, Akihiko Kosugi
Aug 5, 2003·Journal of Bacteriology·Sandrine PagèsJean-Pierre Bélaïch
Aug 19, 2003·Journal of Bacteriology·Tali W DrorYuval Shoham
Oct 4, 2003·Journal of Bacteriology·Roy H DoiSung Ok Han
Apr 13, 2012·Biotechnology for Biofuels·Fernando SegatoRolf A Prade
Dec 22, 2007·Annals of the New York Academy of Sciences·Roy H Doi
Oct 19, 2004·Annual Review of Microbiology·Edward A BayerRaphael Lamed
Jul 11, 2019·International Journal of Molecular Sciences·Ying WangShao-Yuan Leu

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