Determination of sulfonylurea herbicides in water and food samples using sol-gel glass-based immunoaffinity extraction and liquid chromatography-ultraviolet/diode array detection or liquid chromatography-tandem mass spectrometry

Journal of Agricultural and Food Chemistry
Petra DegelmannD Knopp

Abstract

Immunoaffinity supports (IAS) were prepared using broad specific polyclonal anti-sulfonylurea (SU) antibodies immobilized in sol-gel glass. Two different kinds of supports were applied, crushed sol-gel monoliths and sol-gel-coated highly porous silica particles. Both were used for the quantitative enrichment of SUs in natural water and food samples followed by high-performance liquid chromatography-ultraviolet/diode array detection (HPLC-UV/DAD) and tandem mass spectrometry (LC-MS/MS), respectively. Loading, washing, and elution conditions of IAS were optimized. The capacity of supports was determined for 30 SUs and compared with the cross-reactivity pattern of the direct competitive enzyme-linked immunosorbent assay. The capacities correlated well with the affinity to individual SU compounds. Even analytes to which the polyclonal antibodies showed only a lower cross-reactivity could be enriched to a certain degree, if a sufficient capacity of IAS was provided. The IAS could be reused at least 10 times without a loss of effectiveness. Recovery of 16 selected SUs extracted from spiked water and food samples was dependent on the affinity of both immobilized antibodies to single compounds and matrix interferences. In water, 13 SUs...Continue Reading

Citations

May 23, 2006·Analytical and Bioanalytical Chemistry·Dietmar Knopp
Jan 13, 2010·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Fei DingYing Sun
Sep 9, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Christine KlinglmayrMargit Cichna-Markl
Jul 4, 2009·Journal of Chromatography. a·Zdenka Brenn-StruckhofovaEbrahim Razzazi-Fazeli
Jan 26, 2010·Journal of Separation Science·Kishore K R Tetala, Teris A van Beek
Jul 18, 2006·Journal of Chromatography. a·Margit Cichna-Markl
Mar 30, 2018·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Zhe ZhangJin L Zhang
Dec 1, 2006·Journal of Mass Spectrometry : JMS
Jun 2, 2007·Analytical and Bioanalytical Chemistry·Dimitra A Lambropoulou, Triantafyllos A Albanis
Oct 16, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Anna-Cathrine NeumannDietmar Knopp

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