PMID: 9530558Apr 8, 1998Paper

Determination of T-lymphocyte adhesion to endothelial cells using a novel luminescence marker

Immunotechnology : an International Journal of Immunological Engineering
C C TermeerJ Simon

Abstract

Here we report the quantification of T cell adhesion to endothelial cells using the luminescence marker BioLite. A new application for this substance was established using a microassay for the detection of TK-1 mouse T-lymphoma cell adhesion to unstimulated or TNF alpha-stimulated eEnd.2 mouse vascular endothelioma cells. Prelabelling of TK-1 with the marker resulted in luminescence values linearly related to the number of adherent T cells. The marker was not toxic for T cells or endothelial cells nor did it interfere with the expression or function of adhesion molecules on T cells (LFA-1, VLA-4) or endothelial cells (ICAM-1, VCAM-1). When compared with established techniques to quantify T cell/endothelial cell adhesion, i.e. microscopical evaluation or isotope prelabelling of T cells, this method was found to be just as reliable and sensitive.

Citations

Sep 30, 1999·Microscopy Research and Technique·R CrétonL F Jaffe
Dec 9, 1998·Clinical and Experimental Immunology·C C TermeerJ C Simon
Feb 6, 2013·Journal of Plant Research·Chuan-Liang DengLong-Dou Lu

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