May 20, 1992

Determination of tannic acid and its phenolic metabolites in biological fluids by high-performance liquid chromatography

Journal of Chromatography
J ZhuL J Filippich

Abstract

A method for the identification and determination of tannic acid and its phenolic metabolites in biological fluids by high-performance liquid chromatography was developed. Tannic acid and four phenolic compounds, namely gallic acid, pyrogallol, 4-O-methylgallic acid and ellagic acid, were successfully extracted from the biological fluids by using ethyl acetate at acidic conditions. Gallic acid, pyrogallol and 4-O-methylgallic acid were found in the sheep urine, gallic acid, 4-O-methylgallic acid and ellagic acid in plasma, and gallic acid and ellagic acid in abomasal fluid after abomasal dosing of tannic acid. Tannic acid was found in the plasma apart from the abomasal fluid into which it was administered. The concentrations of tannic acid, gallic acid, pyrogallol, 4-O-methylgallic acid and ellagic acid in plasma, abomasal fluid and urine were measured. This method could be applied to measurement of other hydrolysable tannins and their phenolic metabolites in biological materials.

  • References3
  • Citations17

References

  • References3
  • Citations17

Citations

Mentioned in this Paper

Abomasum
Dall Sheep
Spectrophotometry, Ultraviolet
High Pressure Liquid Chromatography Procedure
Body Fluids
Phenols
Pyrogallol Tannins
Ellagitannins
Hydrolysis
Metazoa

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