Determination of tetramine in marine gastropods by liquid chromatography/electrospray ionization-mass spectrometry

Toxicon : Official Journal of the International Society on Toxinology
Yoko KawashimaK Shiomi

Abstract

Tetramine (tetramethylammonium ion) is found at high levels (several mg/g) in the salivary gland of buccinid gastropods and has been involved in numerous poisoning incidents after ingestion of those gastropods. A sensitive and selective determination method for tetramine, which is based on a combination of liquid chromatography (LC) and electrospray ionization-single quadrupole mass spectrometry (ESI-MS), was developed. Following separation by LC on a cation-exchange column, tetramine was easily detected by simultaneous monitoring of a molecular ion (m/z 74) at a cone voltage of 30 V and a fragment ion (m/z 58) at 70 V. A linear calibration curve was obtained in the range of 0.1-100 ng by plotting the peak areas of the molecular ion versus the amounts of tetramine. Spiking experiments demonstrated that tetramine in gastropod tissues can be determined by the LC/ESI-MS method, without being affected by sample matrices as well as the extration procedure. Applications of the new method to gastropod samples revealed that a small amount of tetramine is contained even in mid-gut gland and muscle and that tetramine in the salivary gland diffuses to other tissues during boiling and slow thawing.

References

Jan 1, 1989·Toxicon : Official Journal of the International Society on Toxinology·U AnthoniC Christophersen
Dec 23, 1983·Journal of Chromatography·H SaitohK Kamimura
Mar 15, 2003·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Yoko KawashimaKazuo Shiomi

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Citations

Jan 27, 2005·Journal of Mass Spectrometry : JMS
Jan 19, 2021·Journal of General and Family Medicine·Takashi WatariYu Amano

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