Determination of the active form of the tetranuclear copper sulfur cluster in nitrous oxide reductase

Journal of the American Chemical Society
Esther M JohnstonEdward I Solomon

Abstract

N2OR has been found to have two structural forms of its tetranuclear copper active site, the 4CuS Cu(Z)* form and the 4Cu2S Cu(Z) form. EPR, resonance Raman, and MCD spectroscopies have been used to determine the redox states of these sites under different reductant conditions, showing that the Cu(Z)* site accesses the 1-hole and fully reduced redox states, while the Cu(Z) site accesses the 2-hole and 1-hole redox states. Single-turnover reactions of N2OR for Cu(Z) and Cu(Z)* poised in these redox states and steady-state turnover assays with different proportions of Cu(Z) and Cu(Z)* show that only fully reduced Cu(Z)* is catalytically competent in rapid turnover with N2O.

References

Mar 4, 2000·Nature Structural Biology·K BrownC Cambillau
Dec 18, 2003·Journal of the American Chemical Society·Somdatta GhoshEdward I Solomon
Mar 12, 2004·Journal of the American Chemical Society·Jeannine M ChanDavid M Dooley
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Apr 28, 2010·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Simone Dell'AcquaIsabel Moura
Mar 28, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Simone Dell'AcquaIsabel Moura

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Citations

Mar 11, 2016·Current Opinion in Chemical Biology·Robert R EadyS Samar Hasnain
Sep 30, 2016·Journal of the American Chemical Society·Brittany J JohnsonNeal P Mankad
Apr 11, 2017·Chemical Reviews·Claudia CoughlanKevin M Ryan
Mar 5, 2014·Chemical Reviews·Edward I SolomonLi Tian
Jul 22, 2016·Dalton Transactions : an International Journal of Inorganic Chemistry·David M Ermert, Leslie J Murray
Jun 26, 2015·Chemical Communications : Chem Comm·Brittany J JohnsonNeal P Mankad
Jul 19, 2018·Angewandte Chemie·Vadim Zhuravlev, Przemysław J Malinowski
Oct 11, 2015·Applied and Environmental Microbiology·Brian J VaccaroMichael W W Adams
Aug 1, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ying-Wu Lin
Aug 28, 2020·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Cíntia CarreiraSofia R Pauleta
Jan 16, 2018·Chemical Communications : Chem Comm·Sharareh Bagherzadeh, Neal P Mankad
Feb 29, 2020·Chemical Reviews·Christina FerousiKyle M Lancaster
Mar 12, 2021·Coordination Chemistry Reviews·Suresh C Rathnayaka, Neal P Mankad
May 6, 2021·Chemical Communications : Chem Comm·Yosuke FukudaTakumi Konno
Feb 15, 2014·Journal of the American Chemical Society·Ming-Li TsaiEdward I Solomon
Dec 31, 2020·Journal of the American Chemical Society·Lin ZhangOliver Einsle
Sep 21, 2017·Journal of Inorganic Biochemistry·Cíntia CarreiraIsabel Moura
Dec 6, 2021·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Peter M H Kroneck

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