Determination of the amino acid substitution in human prothrombin type 3 (157 Glu leads to Lys) and the localization of a third thrombin cleavage site

British Journal of Haematology
P G Board, D C Shaw

Abstract

Prothrombin was purified from normal blood donors and individuals heterozygous for prothrombin type 3. Comparison of the purified prothrombin preparations by tryptic peptide mapping, amino acid analysis and automated sequencing after thrombin digestion, has indicated that prothrombin type 3 results from the substitution of a lysine residue for glutamic acid at position 157. This substitution can result from a single base change in the structural gene and explains the relatively slow electrophoretic mobility of prothrombin type 3 at alkaline pH. An additional thrombin cleavage site in profragment 1 has been identified at arginine 54 by automated sequence analysis of thrombin digests by prothrombin.

References

Jan 1, 1980·Annual Review of Biochemistry·C M Jackson, Y Nemerson
Jan 1, 1982·Annals of Human Genetics·P G BoardM E Pidcock

❮ Previous
Next ❯

Citations

Apr 1, 1991·Baillière's Clinical Haematology·V S BlanchetteC Turner
Apr 5, 1996·The Journal of Biological Chemistry·W K StevensM E Nesheim
Sep 14, 2002·The Journal of Biological Chemistry·Julian V MeltonPeter W Gage
Jan 15, 2016·The Journal of Biological Chemistry·Nicola PozziEnrico Di Cera
Mar 7, 1998·Biochemistry·R J PetrovanJ Rosing

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.