Determination of the antitubercular drug PA-824 in rat plasma, lung and brain tissues by liquid chromatography tandem mass spectrometry: application to a pharmacokinetic study

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Dominika BratkowskaThavendran Govender

Abstract

A selective, sensitive and high performance liquid chromatography-tandem mass spectrometry (LC-(ESI)MS/MS) method has been developed and validated for the quantification of the potent antitubercular drug candidate, PA-824, in rat plasma, lung and brain tissues. Sample clean-up involved protein precipitation and solid-phase extraction. Chromatographic separation was performed on YMC Triart C18 column (150 mm × 3.0 mm, 3.0 μm). The method was validated over the concentration range of 75-1500 ng/mL for plasma, 50-1200 ng/g for lungs and 100-1500 ng/g for brain tissue. Evaluation of the pharmacokinetic properties of PA-824 utilized Sprague Dawley rats with a dosage of 20mg/kg at various time points. The new method was applied successfully for the determination of PA-824 with liquid desorption followed by liquid chromatography with ultra-high resolution quadrupole time-of-flight mass spectrometry in the different biological samples.

Citations

Sep 4, 2015·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Chiedza F MunyezaThavendran Govender
Jul 25, 2015·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Adeola ShoboThavendran Govender
Nov 12, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Libin WangShengyong Zhang
Sep 19, 2015·Drug Testing and Analysis·Adeola ShoboThavendran Govender
Jun 18, 2017·Biomedical Chromatography : BMC·Sipho MdandaThavendran Govender
Jan 27, 2018·Journal of Chromatographic Science·Libin WangShengyong Zhang

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