Determination of the cationic amphiphilic drug-DNA binding mode and DNA-assisted fluorescence resonance energy transfer amplification

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Zahid Yaseen Kabir-ud-Din

Abstract

Understanding the mechanism of drug-DNA binding is crucial for predicting the potential genotoxicity of drugs. Agarose gel electrophoresis, absorption, steady state fluorescence, and circular dichroism have been used in exploring the interaction of cationic amphiphilic drugs (CADs) such as amitriptyline hydrochloride (AMT), imipramine hydrochloride (IMP), and promethazine hydrochloride (PMT) with calf thymus or pUC19 DNA. Agarose gel electrophoresis assay, along with absorption and steady state fluorescence studies, reveal interaction between the CADs and DNA. A comparative study of the drugs with respect to the effect of urea, iodide induced quenching, and ethidium bromide (EB) exclusion assay reflects binding of CADs to the DNA primarily in an intercalative fashion. Circular dichroism data also support the intercalative mode of binding. Besides quenching, there is fluorescence exchange energy transfer (FRET) in between CADs and EB using DNA as a template.

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Citations

May 23, 2015·Journal of Biomolecular Structure & Dynamics·Zahra Aramesh-BoroujeniMeissam Noroozifar
Apr 8, 2015·International Journal of Biological Macromolecules·Mohd Akram Kabir-ud-Din
Apr 17, 2016·Journal of Photochemistry and Photobiology. B, Biology·Mehtab ParveenP S Pereira Silva
Apr 18, 2016·International Journal of Medical Microbiology : IJMM·Jean Henrique de Silva RodriguesMichael Duszenko
May 9, 2015·Archives of Biochemistry and Biophysics·Sayeed Ur RehmanMohammad Tabish
Oct 30, 2016·Journal of Biomolecular Structure & Dynamics·Ting RenJunbiao Chang
Nov 7, 2019·Journal of Biomolecular Structure & Dynamics·Zahra Aramesh-BoroujeniMeissam Noroozifar
Dec 29, 2020·International Journal of Biological Macromolecules·Josival Emanuel Ferreira AlvesSinara Mônica Vitalino de Almeida
May 14, 2021·Heliyon·Zubair Rehman NengrooZeshan Umar Shah
May 31, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Irfan HussainMohammad Tabish
Sep 10, 2020·The Journal of Physical Chemistry. B·Shingo HirashimaSoyoung Park

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