Determination of the charge of the plasma proteins and consequent Donnan equilibrium across the capillary barriers in the rat microvasculature

Acta Physiologica
L RügheimerM Wolgast

Abstract

Due to the negatively charged proteins in plasma, a Donnan equilibrium will be formed between plasma and interstitium or, as in the glomerulus, between glomerular plasma and Bowman's space. The phenomenon is of great physiological significance in the sense that the electro-osmotic pressure offered by the small ions attracted to the proteins may account for an important part of the total colloid osmotic pressure and also as the electric potential consequent to the Donnan distribution will affect the transcapillary transport of all charged molecular compounds. The present study aimed at estimating the protein charge in rat plasma in order to validate its importance for colloid osmotic pressure and potential. The charge of the plasma proteins was determined in vitro from the concentration of sodium across a cellophane membrane separating a rat plasma sample from saline alone. However, in order to improve the sensitivity of the method, the studies were carried out at an ionic strength of 1/10 of physiological saline. The average charge of plasma was estimated at 0.23 +/- 0.003 mEq g(-1) protein (mean +/- SE), and the standard variation at +/-0.01 mEq g(-1), i.e. about 5%. At the normal protein concentration in Wistar rats of 50 g L...Continue Reading

References

Mar 1, 1987·Acta Physiologica Scandinavica·G OjtegM Wolgast
Jun 10, 2000·Journal of AAPOS : the Official Publication of the American Association for Pediatric Ophthalmology and Strabismus·D L Guyton

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Citations

Oct 18, 2011·Seminars in Dialysis·Jochen G RaimannPeter Kotanko
Nov 27, 2008·Acta Physiologica·Helge Wiig
Mar 9, 2017·Journal of Neural Engineering·Alejandro Carnicer-LombarteAnne Vanhoestenberghe
Mar 20, 2013·Electrolyte & Blood Pressure : E & BP·Seoung Woo Lee
Feb 29, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ana PeigneuxSarah Hedtrich
Jan 29, 2011·The Journal of Experimental Biology·Liisa M Peltonen, Satu Sankari

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