Determination of the chromosomal relationship between mecA and gyrA in methicillin-resistant coagulase-negative staphylococci.

Antimicrobial Agents and Chemotherapy
P D FeyGordon L Archer

Abstract

mecA, the gene that mediates methicillin resistance, and its accompanying mec locus DNA, insert near the gyrA gene in Staphylococcus aureus. To investigate whether there is a similar relationship between mecA and gyrA in coagulase-negative staphylococci (CNS), mecA- and gyrA-specific DNA fragments were used to probe methicillin-resistant isolates of Staphylococcus epidermidis (MRSE) (n = 11) and Staphylococcus haemolyticus (MRSH) (n = 11). The gyrA probe hybridized to the same SmaI DNA fragment as the mecA probe in all strains tested. However, since the size of the SmaI fragments containing mecA and gyrA varied from 73 to 600 kb, the distance between the two genes was determined more precisely. Cloned mecA or gyrA fragments plus vector sequences each containing a SmaI site were introduced into the chromosome of three isolates each of MRSE and methicillin-resistant S. aureus (MRSA), and the sizes of the generated SmaI fragments were determined by pulsed-field gel electrophoresis. The distance between gyrA and mecA was found to be between 38 and 42 kb in both MRSE and MRSA, and the two genes were in the same relative orientation in all strains. Restriction fragment length polymorphism (RFLP) patterns around the gyrA gene in CNS w...Continue Reading

References

Nov 1, 1987·Antimicrobial Agents and Chemotherapy·D W GalettoG L Archer
Jun 1, 1981·Analytical Biochemistry·D S Holmes, M Quigley
Jan 1, 1995·Microbiology and Immunology·K Hiramatsu
Mar 1, 1995·Journal of Clinical Microbiology·T L BannermanJ M Miller
Oct 1, 1994·Trends in Microbiology·G L Archer, D M Niemeyer
Mar 1, 1994·Antimicrobial Agents and Chemotherapy·G L ArcherM J Pucci
Apr 1, 1996·Antimicrobial Agents and Chemotherapy·G L ArcherM J Pucci

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Citations

Mar 26, 2003·Antimicrobial Agents and Chemotherapy·Adriana E RosatoGordon L Archer
Dec 25, 2002·Antimicrobial Agents and Chemotherapy·P D FeyP M Schlievert
Jun 22, 2001·Microbes and Infection·R P NovickA Ruzin
Nov 26, 2011·Journal of Clinical Microbiology·Sam BoundyGordon L Archer
May 25, 2005·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·M MiragaiaH de Lencastre
Nov 30, 2019·Annals of Clinical Microbiology and Antimicrobials·Isabela Rotta BatistaValéria Cataneli Pereira
Jun 23, 1998·Diagnostic Microbiology and Infectious Disease·R N Jones, M A Pfaller

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